Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2006
Volume 9
Issue 4
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Piegza M. , Witkowska D. , Robak M. 2006. HYDROLASES OF GEOTRICHUM CANDIDUM YEASTS IN BARLEY BETA-GLUCAN DEGRADATION, EJPAU 9(4), #17.
Available Online: http://www.ejpau.media.pl/volume9/issue4/abs-17.html

HYDROLASES OF GEOTRICHUM CANDIDUM YEASTS IN BARLEY BETA-GLUCAN DEGRADATION

Michał Piegza, Danuta Witkowska, Małgorzata Robak
Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Poland

 

ABSTRACT



The research was focused on the capability of eight Geotrichum candidum strains to produce hydrolases, which can be useful for beta-glucan degradation. The evaluation of synthesis character, in synthetic media with sugar beet pulps and with malt, barley and sugar beet pulp, was checked. The degradation of barley glucan and its monitoring by HPLC of the reaction products was performed.

The yeasts were able to the secrete of enzymes degradation laminarin, lichen and beta-glucan, and the activity of those enzymes reached satisfactory level. The products size of beta-glucan hydrolyze showed the that enzymes of Geotrichum candidum mainly attack the endogenous bonds in glucan structure.

Key words: Geotrichum candidum, laminarinase, lichenase, beta-glucan, HPLC.


Michał Piegza
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: mikey@wnoz.ar.wroc.pl

Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: wit@ozi.ar.wroc.pl

Małgorzata Robak
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wrocław, Poland
email: malgorzata.robak@up.wroc.pl

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