Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2006
Volume 9
Issue 3
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Piegza M. , Barszczewski W. , Witkowska D. , Stempniewicz R. , Robak M. 2006. SCANNING OF GEOTRICHUM CANDIDUM GENOME BY RFLP-PCR OF rDNA AND RAPD, EJPAU 9(3), #21.
Available Online: http://www.ejpau.media.pl/volume9/issue3/abs-21.html

SCANNING OF GEOTRICHUM CANDIDUM GENOME BY RFLP-PCR OF RDNA AND RAPD

Micha³ Piegza, Wojciech Barszczewski, Danuta Witkowska, Regina Stempniewicz, Ma³gorzata Robak
Department of Biotechnology and Food Microbiology, Wroc³aw University of Environmental and Life Sciences, Poland

 

ABSTRACT



In the present work we focused on the comparison of genome diversity of 23 strains of Geotrichum candidum isolated from different sources. The analysis of restriction fragment length polymorphism after amplification of rDNA cluster with NS3-ITS4 primers (RFLP-PCR rDNA) and randomly amplified polymorphic DNA (RAPD) analysis with four primers [(GTG)5, (GAC)5, (GACA)4 and M13] were applied for scanning the genome. In RFLP-PCR rDNA with Msp I the physiological classification at species level was confirmed, however two types of pattern were obtained with Hae III. The diversity among some strains was observed in RAPD. Microsatellite primer (GACA)4 was the most discriminating one, only one species specific product was observed. For 20 strains, primer (GTG)5 gave very similar pattern (>80%) allowing the determination of three species specific products (920, 720 and 510 bp). Species specific products were also found with (GAC)5 and M13 primers. The microsatellite overall dendrogram allowed the individual recognition of five strains of G. candidum, for which the patterns similarity was <70%. RAPD patterns similarity of 80% or more, obtained for the rest of strains, reflected rather low genome diversity of G. candidum.

Key words: Geotrichum candidum, RFLP-PCR, RAPD-PCR.

Micha³ Piegza
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
phone +48 71 320 7737
fax +48 71 320 7794
51-630 Wroc³aw
Poland
email: michal.piegza@upwr.edu.pl

Wojciech Barszczewski
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroc³aw, Poland
Phone: +48 71 3205463
email: wojciech.barszczewski@up.wroc.pl

Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: wit@ozi.ar.wroc.pl

Regina Stempniewicz
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroc³aw, Poland
Fax. 4871- 3284124
Phone: 48-71-3205116

Ma³gorzata Robak
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: malgorzata.robak@up.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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