EJPAU 2006. Zimecki M. , Artym J. , Chodaczek G. , Kocięba M. , Rybka J. , Skórska A. , Kruzel M. GLYCOMACROPEPTIDE PROTECTS AGAINST EXPERIMENTAL ENDOTOXEMIA AND BACTEREMIA IN MICE

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2006
Volume 9
Issue 2

Topic:

Veterinary Medicine

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Zimecki M. , Artym J. , Chodaczek G. , Kocięba M. , Rybka J. , Skórska A. , Kruzel M. 2006. GLYCOMACROPEPTIDE PROTECTS AGAINST EXPERIMENTAL ENDOTOXEMIA AND BACTEREMIA IN MICE, EJPAU 9(2), #12.
Available Online: http://www.ejpau.media.pl/volume9/issue2/abs-12.html

GLYCOMACROPEPTIDE PROTECTS AGAINST EXPERIMENTAL ENDOTOXEMIA AND BACTEREMIA IN MICE

Michał Zimecki¹, Jolanta Artym², Grzegorz Chodaczek², Maja Kocięba¹, Jacek Rybka², Anna Skórska², Marian Kruzel³
¹ Institute of Immunology and Experimental Therapy of Polish Academy of Sciences, Wrocław, Poland
² Laboratory of Immunobiology, The Institute of Immunology and Experimental Therapy, Wrocław, Poland
³ University of Texas, Health Science Center, Houston, TX, USA

ABSTRACT

The aim of this study was to evaluate protective effects of glycomacropeptide (GMP), a kappa casein-derived peptide, in experimentally induced endotoxemia or bacteremia in mice. The results showed that BALB/c mice, given intraperitoneally (i.p.) GMP, 24h before intravenous (i.v.) injection of a high dose of lipopolysaccharide (LPS) from Escherichia coli, strongly inhibited serum levels of tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL-6), measured 2h later by bioassays. In addition, GMP, administered 24h before infection of CBA mice with a sublethal dose of E. coli, significantly lowered the number of bacterial cells in the spleen. The analysis of main blood cell types in mice pretreated 24h prior to infection with GMP revealed significant increase in the content of granulocytes and immature neutrophils. We, therefore, postulate, that induction of myelopoiesis by GMP may be a primary cause of the increased clearance of bacteria during the development of bacteremia in mice.

Key words: glycomacropeptide, endotoxemia, bacteremia, granulocytes.

Michał Zimecki
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland
email: zimecki@immuno.iitd.pan.wroc.pl

Jolanta Artym
Laboratory of Immunobiology,
The Institute of Immunology and Experimental Therapy, Wrocław, Poland

Grzegorz Chodaczek
Laboratory of Immunobiology,
The Institute of Immunology and Experimental Therapy, Wrocław, Poland

Maja Kocięba
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland

Jacek Rybka
Laboratory of Immunobiology,
The Institute of Immunology and Experimental Therapy, Wrocław, Poland

Anna Skórska
Laboratory of Immunobiology,
The Institute of Immunology and Experimental Therapy, Wrocław, Poland

Marian Kruzel
University of Texas, Health Science Center, Houston, TX, USA

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.