Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Rywińska A. , Wojtatowicz M. , Rymowicz W. 2006. CITRIC ACID BIOSYNTHESIS BY YARROWIA LIPOLYTICA A-101-1.31 UNDER DEFICIENCY OF VARIOUS MEDIUM MACROCOMPONENTS, EJPAU 9(1), #15.
Available Online: http://www.ejpau.media.pl/volume9/issue1/abs-15.html

ABSTRACT

The objective of the study was to examine the potential of Yarrowia lipolytica A-101-1.31 growth control via the concentration of the nitrogen, phosphorus or sulphur source, as well as to assess the efficiency of each source separately and under simultaneous deficiency of nitrogen and phosphorus.

The experimental study has produced the following findings: nitrogen, phosphorus and sulphur enabled the control of Y.lipolytica A-101-1.31 growth. Citric acid biosynthesis occurred irrespective of the growth-limiting factor used. The yeast cells produced citric acid with the desired efficiency only under conditions of nitrogen (q_CA^g=0.071 gg^-1h^-1) or sulphur (Y_CA^P=0.74 gg^-1) deficiency. The smallest changes in the elemental composition were observed when the yeast grew under nitrogen limitation. Elemental composition variations were the most distinct when the yeast biomass was cultivated under phosphorus deficiency. A low deficiency of phosphorus in the nitrogen deficient had a stimulating effect on the acid-producing of the growing cells (q_CA^g=0.089 gg^-1h^-1).

Key words: citric acid, Yarrowia lipolytica, growth limitation.

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Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: anita.rywinska@wnoz.up.wroc.pl

Maria Wojtatowicz
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
C.K. Norwida 25, 50-375 Wrocław, Poland
Fax. 4871- 3284124
Phone: 48-71-3205117
email: mwojt@wnoz.ar.wroc.pl

Waldemar Rymowicz
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroclaw, Poland
Fax. 48-71-3284124
Phone: 48-71-3205143
email: rymowicz@ozi.ar.wroc.pl

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