EJPAU 2006. Matyka S. , Rubaj J. , Korol W. , Bielecka G. INDUSTRIAL FEED MIXTURES WITH AN ADDITION OF METHIONINE HYDROXY ANALOG

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2006
Volume 9
Issue 1

Topic:

Agricultural Engineering

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Matyka S. , Rubaj J. , Korol W. , Bielecka G. 2006. INDUSTRIAL FEED MIXTURES WITH AN ADDITION OF METHIONINE HYDROXY ANALOG, EJPAU 9(1), #12.
Available Online: http://www.ejpau.media.pl/volume9/issue1/abs-12.html

INDUSTRIAL FEED MIXTURES WITH AN ADDITION OF METHIONINE HYDROXY ANALOG

Stanisław Matyka¹, Jolanta Rubaj², Waldemar Korol², Grażyna Bielecka²
¹ Department of Biological Bases of Food and Feed Technology, Agricultural University in Lublin, Poland
² National Laboratory of Feeds in Lublin, Poland

ABSTRACT

HMB (2-hydroxy-4-(methythio)butanoic acid), which is the precursor of methionine in the organism, is widely used in the production of industrial feeds as an addition supplementing the deficiency of methionine proper. HMB does not have any amino group, which is the reason why it cannot be marked within the analysis of the aminoacidic composition using the reaction with ninhydrine reagent. In the method of marking HMB using HPLC technique the conditions of chromatographic division were modified. The applied system of chromatographic columns and movable and eluating phases enabled effective separation of HMB from the elements of differentiated matrixes of industrial feed mixtures. The analytical proceedings are characterized by good validation parameters, i.e. high selectivity, high repeatability and a high degree of recovery of contents occurring in the production practice of industrial feeds ranging from 0.05 to 0.4%.

Key words: industrial feed mixtures, methionine hydroxyl, determination method.

Stanisław Matyka
Department of Biological Bases of Food and Feed Technology,
Agricultural University in Lublin, Poland
48 Doświadczalna Street, 20-236 Lublin, Poland
tel./fax. (+48 81) 461 05 62
email: smatyka@onet.pl

Jolanta Rubaj
National Laboratory of Feeds in Lublin, Poland
2 Chmielna Street, 20-097 Lublin, Poland

Waldemar Korol
National Laboratory of Feeds in Lublin, Poland
2 Chmielna Street, 20-097 Lublin, Poland

Grażyna Bielecka
National Laboratory of Feeds in Lublin, Poland
2 Chmielna Street, 20-097 Lublin, Poland

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