Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 4
Topic:
Veterinary Medicine
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Wójcik M. , Bobowiec R. , Klimont M. , Tusińska E. 2005. EFFECT OF 17β-ESTRADIOL ON GLUCOSE TOLERANCE AND PLASMA INSULIN CONCENTRATION IN ANESTRAL SHEEP, EJPAU 8(4), #33.
Available Online: http://www.ejpau.media.pl/volume8/issue4/abs-33.html

EFFECT OF 17β-ESTRADIOL ON GLUCOSE TOLERANCE AND PLASMA INSULIN CONCENTRATION IN ANESTRAL SHEEP

Marta Wójcik, Ryszard Bobowiec, Michał Klimont, Elżbieta Tusińska
Department of Pathophysiology Chair of Preclinical Veterinary Sciences, Agricultural Academy in Lublin, Poland

 

ABSTRACT



Natural estrogens especially 17β-estradiol has been implicated as etiologic factors in develop of disturbances of insulin releasing and glucose metabolism. Experiments were carried out on 6 Polish Lowland sheep, weighing between 40-42 kg, 1.5 years of age each. The animals were divided into two groups: I – control group; II – intramuscularly treatment with 17β-estradiol 5mg/kg b wt. At the sixth day from the beginning of estradiol treatment, in the both group of animals intravenous glucose tolerance test was carried out. After 5-days administration of estradiol, plasma concentration of this hormone was significantly (p£0.05) higher in comparison to the control and reached maximum value 125.88±2.96 pg/ml. In the control group, the infusion of hypertonic solution of glucose increased the plasma glucose concentration from 3.16±0.15 mM/l to maximum 25.59± 1.49 mM/l in 10 min and produced marked rise in the concentration of plasma insulin from 229.76 pM/l to 1173.68 pM/l. In comparison to the control, infusion of glucose in estrogen-treatment group of sheep caused substantial rise of glucose level to maximum 24.47±3.29 mM/l in 30 min. This considerable increase of glucose concentration was maintained during the next 90 minutes of experiments. The insulin response to i.v. glucose infusion in this group of animals, was reduced and did not exceed 641.62±6.44 pM/l.

Key words: 17β-estradiol, glucose, insulin, sheep.


Marta Wójcik
Department of Pathophysiology Chair of Preclinical Veterinary Sciences,
Agricultural Academy in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland

email: marta.wojcik@agros.ar.lublin.pl

Ryszard Bobowiec
Department of Pathophysiology Chair of Preclinical Veterinary Sciences,
Agricultural Academy in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland

Michał Klimont
Department of Pathophysiology Chair of Preclinical Veterinary Sciences,
Agricultural Academy in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland

Elżbieta Tusińska
Department of Pathophysiology Chair of Preclinical Veterinary Sciences,
Agricultural Academy in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland

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