Volume 8
Issue 3
Food Science and Technology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume8/issue3/abs-18.html
PHASE TRANSITIONS OF POTATO STARCH HYDROLYSATES DIFFERING IN BRANCHING CHARACTERISTICS
Rafał Ziobro1, Halina Gambu¶1, Dorota Gumul1, Werner Praznik2, Tomasz Jankowski3
1 Department of Carbohydrates Technology,
Agricultural University of Cracow, Poland
2 Institute of Chemistry,
University of Agricultural Sciences, Wien, Austria
3 Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
ABSTRACT
In order to establish the influence of branching characteristics on thermal properties of starch hydrolysates, phase transitions of samples differing in content of amylose have been compared. Potato starch dissolved in DMSO/water mixture was hydrolysed by crude pullulanase preparation and fractionated by precipitation. The fractions differed in their branching characteristics and after solubilisation and retrogradation gave endotherms at different temperatures. The results prove that even slight changes in branching pattern can cause significant variation in phase transitions. The solvent system used in the experiments seems to stabilize linear glucans for a long time and promote recrystallisation of branched molecules.
Key words: starch hydrolysates, enzymatic hydrolysis .
Rafał Ziobro
Department of Carbohydrates Technology,
Agricultural University of Cracow, Poland
Balicka 122, 30-149 Cracow, Poland
email: rrziobro@cyf-kr.edu.pl
Halina Gambu¶
Department of Carbohydrates Technology,
Agricultural University of Cracow, Poland
Balicka 122, 30-149 Cracow, Poland
Dorota Gumul
Department of Carbohydrates Technology,
Agricultural University of Cracow, Poland
Balicka 122, 30-149 Cracow, Poland
Phone: (+48 12) 662 47 71
Fax: (+48 12) 662 47 47
email: rrgumul@cyf-kr.edu.pl
Werner Praznik
Institute of Chemistry,
University of Agricultural Sciences, Wien, Austria
Muthgasse 18, A-1190 Wien, Austria
Tomasz Jankowski
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: 061 846 60 04
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