EJPAU 2005. Bogusławska-Wąs E. , Czeszejko K. , Czekajło-Kołodziej U. , Mędrala D. INTRASPECIES DIFFERENTATION OF <I>SACCHAROMYCES CEREVISIAE </I>STRAINS ISOLATED FROM FISH AND THE ODRA ESTUARY

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2005
Volume 8
Issue 3

Topic:

Biotechnology

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Bogusławska-Wąs E. , Czeszejko K. , Czekajło-Kołodziej U. , Mędrala D. 2005. INTRASPECIES DIFFERENTATION OF SACCHAROMYCES CEREVISIAE STRAINS ISOLATED FROM FISH AND THE ODRA ESTUARY, EJPAU 8(3), #16.
Available Online: http://www.ejpau.media.pl/volume8/issue3/abs-16.html

INTRASPECIES DIFFERENTATION OF SACCHAROMYCES CEREVISIAE STRAINS ISOLATED FROM FISH AND THE ODRA ESTUARY

Elżbieta Bogusławska-Wąs, Katarzyna Czeszejko, Urszula Czekajło-Kołodziej, Dagmara Mędrala
Department of Food Microbiology, Agricultural University of Szczecin, Poland

ABSTRACT

Saccharomyces cerevisiae strains isolated from fish and subsurface layers of the Odra Estuary waters were examined. It was evaluated that the increase in faecal contamination of water samples is related to the increase in the total number of yeasts and yeast-like organisms, including S. cerevisiae. RAPD-PCR analysis enabled to distinguish 5 groups of isolates presenting a high level of similarity of their genetic fingerprints. Evaluation of S. cerevisiae cell wall hydrophobic properties, conducted with the MATH method, revealed occurrence of three separate phenotypic groups which expressed the tested property at different level. 87.5% of hydrophobic strains originated from the most polluted area. No correlation between genotypes and hydrophobicity of strains was observed.

Key words: S. cerevisiae, hydrophobicity, randomly amplified polymorphic DNA (RAPD-PCR) technique, water.

Elżbieta Bogusławska-Wąs
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland
email: ewas@tz.ar.szcecin.pl

Katarzyna Czeszejko
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland

Urszula Czekajło-Kołodziej
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland

Dagmara Mędrala
Department of Food Microbiology,
Agricultural University of Szczecin, Poland
Papieża Pawa VI 3, Szczecin, Poland

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