Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 2
Topic:
Environmental Development
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Nowak J. , Smolik B. , Zakrzewska H. 2005. RELATIONS BETWEEN FLUORINE CONTENT IN SOIL AND INHIBITION OF SOIL ENZYMES ACTIVITY, EJPAU 8(2), #15.
Available Online: http://www.ejpau.media.pl/volume8/issue2/abs-15.html

RELATIONS BETWEEN FLUORINE CONTENT IN SOIL AND INHIBITION OF SOIL ENZYMES ACTIVITY

Janina Nowak1, Beata Smolik2, Helena Zakrzewska1
1 Department of Biochemistry, Agriculture University of Szczecin, Poland
2 Department of Biochemistry, West Pomeranian University of Technology

 

ABSTRACT



In this study the influence of various amounts of fluorine added to soil as NaF solutions (10, 30 and 50 mmol kg-1) on the activity of enzymes (acid and alkaline phosphatase, β-glukosidase and dehydrogenases) in soils of various texture and organic carbon content was tested. Also the effect of aqueous solutions of fluoride added to samples of various kinds of soil on the content of fluorine soluble in 0.01 M CaCl2 and in 2 M HClO4 (potentially available for plants), has been studies.

The laboratory experiment was conducted at 20°C, and 60% water holding capacity, on three kinds of soil which differed in organic carbon content and mechanical composition. For three months the activity of enzymes and content of the two forms of fluorine were analyzed every few days. As reference served soil without any addition of fluoride.

The addition of various doses of NaF to the soil caused an appearance diverse amount of fluorine forms soluble in 0.01 M CaCl2 and in 2 M HClO4. The level of these fluorine forms was connected both with the kind of soil (the lightest soil contained the highest amount of water soluble fluorine), and the amount of the added NaF, and also duration of the experiment. The content of the fluorine forms was related to the inhibition of the tested enzymes. The highest inhibition pertained to the phosphatase, in the sequence: alkaline, acid then β-glukosidase and dehydrogenases. In the latter case a temporary increase of their activity also occurred. A high correlation between the content of the various forms of fluorine in soil and inhibition of the enzymes was found, the highest significance of that relation pertained to β-glukosidase.

Key words: soils, water soluble and insoluble fluorine content, inhibition of soil enzyme activities: phosphatases, β-glukosidase, dehydrogenases.

Janina Nowak
Department of Biochemistry,
Agriculture University of Szczecin, Poland
Słowackiego 17, 71-434 Szczecin, Poland
email: jnowak@agro.ar.szczecin.pl

Beata Smolik
Department of Biochemistry, West Pomeranian University of Technology
Słowackiego St. 17,
71-434 Szczecin, Poland
email: bsmolik@agro.ar.szczecin.pl

Helena Zakrzewska
Department of Biochemistry,
Agriculture University of Szczecin, Poland
Słowackiego 17, 71-434 Szczecin, Poland
email: hzakrzewska@agro.ar.szczecin.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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