Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 2
Topic:
Horticulture
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Lema-Rumińska J. , Zalewska M. , Sadoch Z. , Jerzy M. 2005. IDENTIFICATION OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) MUTANTS OF NERO AND WONDER GROUPS USING RAPD MARKERS, EJPAU 8(2), #04.
Available Online: http://www.ejpau.media.pl/volume8/issue2/abs-04.html

IDENTIFICATION OF CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORA TZVELEV) MUTANTS OF NERO AND WONDER GROUPS USING RAPD MARKERS

Justyna Lema-Rumińska1, Małgorzata Zalewska2, Zbigniew Sadoch3, Marek Jerzy4
1 Department of Ornamental Plants and Vegetable Crops, University of Technology and Agriculture in Bydgoszcz, Poland
2 Department of Ornamental Plants and Vegetable Crops, University of Technology and Life Sciences in Bydgoszcz, Poland
3 Plant Breeding and Acclimatization Institute in Bydgoszcz
4 Department of Ornamental Plants, Agricultural University of Poznan, Poland

 

ABSTRACT



The present study investigated chrysanthemum radiomutants (Dendranthema grandiflora Tzvelev) and their original cultivars of two cultivar groups; Nero and Wonder. The Ney similarity index revealed a high diversity between the cultivars and in all of the cases was less than 0.829. The cluster analysis separated cultivars into three groups, with the one of Nero group, the other of Wonder group and the cultivar 'Bronze Wonder'.

The aim of the present study was to identify cultivars using RAPD markers. Eight of twenty primers resulted in polymorphic bands which allowed for distinguishing between the chrysanthemum cultivars examined. A single primer or set of two primers resulted in a specific fixed and repeatable pattern of bands in each original cultivar and in each mutant - DNA-fingerprinting.

Key words: Dendranthema grandiflora, radiomutants, RAPD, DNA-fingerprinting, identification.


Justyna Lema-Rumińska
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Agriculture in Bydgoszcz, Poland
6 Bernardynska Street, 85-029 Bydgoszcz, Poland
tel. (+48 52) 374 95 22
fax (+48 52) 374-95-05
email: lem-rum@atr.bydgoszcz.pl

Małgorzata Zalewska
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 36
email: zalewska@utp.edu.pl

Zbigniew Sadoch
Plant Breeding and Acclimatization Institute in Bydgoszcz
10 Powstańców Wielkopolskich Street, 85-950 Bydgoszcz, Poland

Marek Jerzy
Department of Ornamental Plants,
Agricultural University of Poznan, Poland
al. D±browskiego 159, 60-594 Poznan, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.