Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Miszkiewicz H. , Bizukojc M. , Rozwandowicz A. , Bielecki S. 2005. STUDIES ON THE PRODUCTION OF PHASEOLUS VULGARIS TEMPE IN DISCONTINUOUSLY ROTATING BIOREACTOR, EJPAU 8(2), #01.
Available Online: http://www.ejpau.media.pl/volume8/issue2/abs-01.html

STUDIES ON THE PRODUCTION OF PHASEOLUS VULGARIS TEMPE IN DISCONTINUOUSLY ROTATING BIOREACTOR

Hanna Miszkiewicz1, Marcin Bizukojc2, Anita Rozwandowicz1, Stanisław Bielecki1
1 Institute of Technical Biochemistry, Technical University of Lodz
2 Department of Bioprocess Engineering, Technical University of Lodz

 

ABSTRACT



Phaseolus vulgaris fermentation was conducted in a discontinuously rotating bioreactor (DRB). Effects of rotation frequency and the bean mass thickness on Rhizopus oligosporus growth and biosynthesis of selected hydrolases were examined. Morphology and physiology of the fungus were estimated by using the digital image analysis. The results were compared with that of the reference stationary process. The fastest growth of R. oligosporus and the most efficient synthesis of selected hydrolases were achieved for the bed (hydrated, acidified, dehulled, and autoclaved cotyledons of P. vulgaris) thickness of 6 cm, and the frequency of agitation of 1 min every 4 h. Solid substrate fermentation in DRB was shown to be superior to that conducted under stationary culture conditions because R. oligosporus hyphae displayed higher physiological activity and the activity of extracellular hydrolases was 20% larger. In consequence, tempe produced in DRB under optimized conditions contained twice more reducing sugars and 25% more soluble proteins as compared to the reference sample.

Key words: solid-substrate fermentation, discontinuously rotating bioreactor, Phaseolus vulgaris, tempe, Rhizopus oligosporus, digital image analysis.


Hanna Miszkiewicz
Institute of Technical Biochemistry,
Technical University of Lodz
ul. Stefanowskiego 4/10, 90-924 Lodz, Poland
email: hamisz@snack.p.lodz.pl

Marcin Bizukojc
Department of Bioprocess Engineering,
Technical University of Lodz
ul. Wolczanska 213/215, 90-924 Lodz, Poland

Anita Rozwandowicz
Institute of Technical Biochemistry,
Technical University of Lodz
ul. Stefanowskiego 4/10, 90-924 Lodz, Poland

Stanisław Bielecki
Institute of Technical Biochemistry,
Technical University of Lodz
ul. Stefanowskiego 4/10, 90-924 Lodz, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.