Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 1
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Piegza M. , Witkowska D. , Stempniewicz R. , Rywińska A. 2005. GEOTRICHUM HYDROLYTIC ACTIVITY IN MILLED MALT AND BARLEY MEDIUM, EJPAU 8(1), #15.
Available Online: http://www.ejpau.media.pl/volume8/issue1/abs-15.html

GEOTRICHUM HYDROLYTIC ACTIVITY IN MILLED MALT AND BARLEY MEDIUM

Micha³ Piegza, Danuta Witkowska, Regina Stempniewicz, Anita Rywińska
Department of Biotechnology and Food Microbiology, Wroc³aw University of Environmental and Life Sciences, Poland

 

ABSTRACT



In present work we focus on biosynthesis of extracellular hydrolase by Geotrichum candidum yeasts, which can be useful in degradation of non-starch polisaccharide (cellulase, xylanase, poligalacturonase, beta-1,3-glucanase, beta-1,3(1,4)-glucanase) and also protein (protease) and acyloglicerole (lipase). As a growth medium, we use 4% milled barley or 4% milled malt suspension. We observed divergence in level of activity of hydrolase, between different strains of Geotrichum but also in used medium. The hydrolase biosynthesis by Geotrichum was much more effective in the medium containing malt than in the one with barley. This effect was not observed for lipase and protease. Strains: G.candidum PH1 and G.candidum 1 due to very high non-starch polisaccharide hydrolase activity and low protease and lipase activity could be proposed as components of starter cultures, useful in malting process.

Key words: Geotrichum candidum, cellulases, xylanases, laminarinases, lichenases, proteases, lipases.


Micha³ Piegza
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: mikey@wnoz.ar.wroc.pl

Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: wit@ozi.ar.wroc.pl

Regina Stempniewicz
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroc³aw, Poland
Fax. 4871- 3284124
Phone: 48-71-3205116

Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: anita.rywinska@wnoz.up.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.