Volume 8
Issue 1
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume8/issue1/abs-15.html
GEOTRICHUM HYDROLYTIC ACTIVITY IN MILLED MALT AND BARLEY MEDIUM
Micha³ Piegza, Danuta Witkowska, Regina Stempniewicz, Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
ABSTRACT
In present work we focus on biosynthesis of extracellular hydrolase by Geotrichum candidum yeasts, which can be useful in degradation of non-starch polisaccharide (cellulase, xylanase, poligalacturonase, beta-1,3-glucanase, beta-1,3(1,4)-glucanase) and also protein (protease) and acyloglicerole (lipase). As a growth medium, we use 4% milled barley or 4% milled malt suspension. We observed divergence in level of activity of hydrolase, between different strains of Geotrichum but also in used medium. The hydrolase biosynthesis by Geotrichum was much more effective in the medium containing malt than in the one with barley. This effect was not observed for lipase and protease. Strains: G.candidum PH1 and G.candidum 1 due to very high non-starch polisaccharide hydrolase activity and low protease and lipase activity could be proposed as components of starter cultures, useful in malting process.
Key words: Geotrichum candidum, cellulases, xylanases, laminarinases, lichenases, proteases, lipases.
Micha³ Piegza
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
phone +48 71 320 7737
fax +48 71 320 7794
51-630 Wroc³aw
Poland
email: michal.piegza@upwr.edu.pl
Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: wit@ozi.ar.wroc.pl
Regina Stempniewicz
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroc³aw, Poland
Fax. 4871- 3284124
Phone: 48-71-3205116
Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: anita.rywinska@wnoz.up.wroc.pl
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.