Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2005
Volume 8
Issue 1
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Kulik T. , Pszczółkowska A. , Olszewski J. , Fordoński G. , Płodzień K. , Sawicka-Sienkiewicz E. 2005. IDENTIFICATION OF Colletotrichum acutatum FROM YELLOW AND ANDEAN LUPIN SEEDS USING PCR ASSAY, EJPAU 8(1), #02.
Available Online: http://www.ejpau.media.pl/volume8/issue1/abs-02.html

IDENTIFICATION OF COLLETOTRICHUM ACUTATUM FROM YELLOW AND ANDEAN LUPIN SEEDS USING PCR ASSAY

Tomasz Kulik1, Agnieszka Pszczółkowska1, Jacek Olszewski1, Gabriel Fordoński1, Krystyna Płodzień1, Ewa Sawicka-Sienkiewicz2
1 Department of Diagnostics and Plant Pathophysiology, University of Warmia and Mazury in Olsztyn
2 Department of Plant Breeding and Seed Production, Agricultural University of Wrocław

 

ABSTRACT



PCR assays were used to identify Colletotrichum acutatum and C. gloeosporioides out of 14 fungal cultures obtained from seed samples of selected yellow and Andean lupin cultivars. The results allowed for a classification of all fungal cultures to C. acutatum. No C. gloeosporioides culture was identified. In addition, the comparison of rDNA sequence fragment from one of the cultures tested with sequences available in the NCBI/GeneBank database confirmed that the species identified was C. acutatum. The BIO-PCR technique was also used to detect potential inoculum of both species in two lupin seed samples. The results of BIO-PCR assays showed that two lupin seed samples were infected with C. acutatum while no isolate of C. gloeosporioides was detected.

Key words: yellow lupin, Andean lupin, anthracnose, Colletotrichum acutatum, Colletotrichum gloeosporioides, BIO-PCR.


Tomasz Kulik
Department of Diagnostics and Plant Pathophysiology,
University of Warmia and Mazury in Olsztyn
Oczapowskiego 8, 10-719 Olsztyn
email: tomaszkulik@hotmail.com

Agnieszka Pszczółkowska
Department of Diagnostics and Plant Pathophysiology,
University of Warmia and Mazury in Olsztyn
Oczapowskiego 8, 10-719 Olsztyn
email: frodo@uwm.edu.pl

Jacek Olszewski
Department of Diagnostics and Plant Pathophysiology,
University of Warmia and Mazury in Olsztyn
Oczapowskiego 8, 10-719 Olsztyn
email: jacolsz@uwm.edu.pl

Gabriel Fordoński
Department of Diagnostics and Plant Pathophysiology,
University of Warmia and Mazury in Olsztyn
Oczapowskiego 8, 10-719 Olsztyn
email: gford@uwm.edu.pl

Krystyna Płodzień
Department of Diagnostics and Plant Pathophysiology,
University of Warmia and Mazury in Olsztyn
Oczapowskiego 8, 10-719 Olsztyn

Ewa Sawicka-Sienkiewicz
Department of Plant Breeding and Seed Production,
Agricultural University of Wrocław
Cybulskiego 34, 50-205 Wrocław
email: ewasawic@ozi.ar.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.