Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Kobiałka N. , Mularczyk M. , Kosiorowska K. , Pilarska K. , Łaba W. , Piegza M. , Robak M. 2019. NEW STRAINS OF FILAMENTOUS FUNGI ISOLATED FROM CONSTRUCTION MATERIALS
DOI:10.30825/5.ejpau.169.2019.22.1 , EJPAU 22(1), #02.
Available Online: http://www.ejpau.media.pl/volume22/issue1/abs-02.html

ABSTRACT

Filamentous fungi are a large group of eukaryotic organisms capable of growth in different environmental niches. The variety of filamentous fungi occurring on building materials is still increasing. In this paper, 19 strains were isolated from deteriorated walls and floor coverings of houses in Opole and Lower Silesia, Poland. The identification of  fungi growing on construction materials was based on microscopic assessment (according the presence or absence of particular, characteristic structures) and molecular identification by  sequence homology of highly diverse regions of ITS1 and ITS2. Obtained isolates were classified into Penicillium, Lecanicillium and Candida genera. Most of the strains (17), have been assigned to the genus Penicillium according to sequence fragments from GenBank Database. The length of the ITS1 region of most isolates was closely related to the length of this region of P. chrysogenum species. The PCR products sequencing confirmed that isolates NK1 and NK2 were P. chrysogenum, the isolate NK3 L. lecanii and theisolateNK12 P. griseoroseum. The isolates Dyw1b, Dyw2, Dyw4, Dyw5b, Dyw6, Dyw7 belonged to the Penicilium genus, however exhibited some differences. The presence of L. lecanii in deteriorated materials was rather surprising but the isolated strain revealed interesting properties. Its spores and biomass are potential biological insecticide agents.

Key words: filamentous fungi, PCR, ITS-region, identification, construction materials.

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Natalia Kobiałka
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Malwina Mularczyk
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Katarzyna Kosiorowska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Kinga M. Pilarska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Wojciech Łaba
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Michał Piegza
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
phone +48 71 320 7737
fax +48 71 320 7794
51-630 Wrocław
Poland
email: michal.piegza@upwr.edu.pl

Małgorzata Robak
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: malgorzata.robak@up.wroc.pl

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Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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