Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2018
Volume 21
Issue 2
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Magnucka E. , Pietr S. 2018. ROLE OF ALKYLRESORCINOLS IN COLONIZATION OF WHEAT BY PSEUDOMONAS SP. STRAIN 150
DOI:10.30825/5.ejpau.18.2018.21.2, EJPAU 21(2), #02.
Available Online: http://www.ejpau.media.pl/volume21/issue2/abs-02.html

ROLE OF ALKYLRESORCINOLS IN COLONIZATION OF WHEAT BY PSEUDOMONAS SP. STRAIN 150
DOI:10.30825/5.EJPAU.18.2018.21.2

Elżbieta G. Magnucka, Stanisław J. Pietr
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland

 

ABSTRACT

Cereals contain 5-n-alkylresorcinols that are potent antimicrobials. Despite of this fact various bacteria effectively colonize cereals. The aim of this study was to determine whether the tolerance of strain PO150, isolated from wheat rhizosphere, to orcinol could play a role in wheat colonization. The formation of the petite-sized and non-fluorescent colonies of this strain was observed already at a dose of 1.0 mg cm-3 orcinol. Its proliferation, in turn, was completely inhibited by 1.5 mg cm-3 of this phenolic compound. A Tn5 mutant with reduced tolerance to orcinol more weakly colonized both wheat kernels and the 24-h-old germinating seeds than the parent strain. Moreover, both the mutant and wild-type strain adhered more effectively to grains of the cultivar which contained less 5-n-alkylresorcinols. Thus, our results suggest that resistance to wheat seed alkylresorcinols can be important for the early stages of its colonization by this rhizobacteria of the genus Pseudomonas.

Key words: colonization, 5-n-alkylresorcinols, Pseudomonas sp., wheat.


Elżbieta G. Magnucka
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
Grunwaldzka 53
50-375 Wrocław
Poland
email: elzbieta.magnucka@upwr.edu.pl

Stanisław J. Pietr
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
phone/fax: +48 71 320 6521
Grunwaldzka 53
50-375 Wrocław
Poland
email: stanislaw.pietr@upwr.edu.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.