Volume 21
Issue 1
Agronomy
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
DOI:10.30825/5.ejpau.15.2018.21.1, EJPAU 21(1), #03.
Available Online: http://www.ejpau.media.pl/volume21/issue1/abs-03.html
FUNGAL BIOSOLUBILIZATION OF POLISH LIGNITE: THE EFFECT OF SUBSTRATE CONCENTRATION
DOI:10.30825/5.EJPAU.15.2018.21.1
Hanna Miszkiewicz, Olga Marchut-Mikołajczyk, Lidia Mielcarz, Stanisław Bielecki
Institute of Technical Biochemistry, Lodz University of Technology, Poland
ABSTRACT
The effect of concentration of lignite, derived from the Polish mine in Bełchatów, pretreated with 8M nitric acid, on the yield of its biosolubilization by Fusarium oxysporum 1101 strain was studied. Observed decrease in pH during lignite biosolubilization by the F. oxysporum 1101 was correlated with amounts of humic and fulvic acids released from the substrate. Conversely, in the lignite-free medium the strain synthesized alkaline compounds that caused an increase in pH. The highest biosolubilization yield was achieved when the culture medium was supplemented with 5% of lignite (3-times higher than in the medium supplemented with 10% of coal). In this case, for the first 8 days, the products with gradually decreasing molecular masses were efficiently released (the E4/E6 ratio was growing) and then polymerized (the E4/E6 ratio was reduced), and the content of condensed aromatic rings was decreased. The FTIR analysis showed that the relative intensity of the peaks reflected the concentration of functional groups in the tested products. Only the spectrum of products released from 10% coal contained the additional peak, corresponding to -CH(Ar) groups.
Key words: Fusarium oxysporum 1101, lignite solubilization, coal loading.
Hanna Miszkiewicz
Institute of Technical Biochemistry, Lodz University of Technology, Poland
B. Stefanowskiego 4/10
90-924 Łódź
Poland
email: hanna.miszkiewicz@p.lodz.pl
Olga Marchut-Mikołajczyk
Institute of Technical Biochemistry, Lodz University of Technology, Poland
B. Stefanowskiego 4/10
90-924 Łódź
Poland
email: olga.marchut-mikołajczyk@p.lodz.pl
Lidia Mielcarz
Institute of Technical Biochemistry, Lodz University of Technology, Poland
B. Stefanowskiego 4/10
90-924 Łódź
Poland
Stanisław Bielecki
Institute of Technical Biochemistry, Lodz University of Technology, Poland
B. Stefanowskiego 4/10
90-924 Łódź
Poland
email: stanisław.bielecki@p.lodz.pl
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.