Volume 20
Issue 4
Horticulture
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
DOI:10.30825/5.ejpau.32.2017.20.4, EJPAU 20(4), #05.
Available Online: http://www.ejpau.media.pl/volume20/issue4/art-05.html
THE EFFECT OF 1-METHYLCYCLOPROPENE AND STORAGE CONDITIONS ON CHOSEN QUALITY TRAITS OF LONG SHELF LIFE (LSL) TOMATO FRUIT, IN RELATION TO GROWING SEASON
DOI:10.30825/5.EJPAU.32.2017.20.4
Anna Wrzodak1, Justyna Szwejda-Grzybowska1, Marek Gajewski2
1 Institute of Horticulture, Poland
2 Warsaw University of Life Sciences – SGGW, Department of Vegetable and Medicinal Plants, Poland
Treatment of horticultural produce with 1-methylcyclopropene (1-MCP) is a
novel method used to extend storage life and to improve postharvest quality of
different fruits and vegetables. In tomato production, several LSL (“Long Shelf Life”)
cultivars with a gene prolonging ripening process, of longer shelf life, were
introduced to practice. It is assumed that 1-MCP treatment of such fruit could
improve its storage ability and shelf life even further. The aim of the 2-year
study was to investigate the effect of 1-MCP treatment on some quality traits
of stored tomato fruit ‘Habana’ F1, the cultivar
of LSL type, in relation to storage temperature. Tomatoes, which were grown on
stakes in open field, were harvested at mature-green stage, treated with 1-MCP
in concentration of 1.0 µL·L-1 and then stored at a cold store under
the two temperature variants (12.5°C and 20°C). There were determined
after 4 weeks of storage: lycopene, total phenolics, ascorbic acid, total sugars
contents, as well as titratable acidity and pH of the fruit. Results of the study
showed big differentiation in chemical composition of the tomato fruit depending
on 1-MCP treatment and storage temperature, but also differences between growing
seasons. The relationship between experimental factors studied was not clear
and differed between the years. Therefore, it can be concluded in general that
the effect of 1-MCP treatment strongly depends on initial physiological state
of tomato fruit, which is modified by environmental conditions during growing
season. After the storage, a tendency to higher content of phenolics for the
fruit treated with 1-MCP was observed. Tomatoes treated with 1-MCP were not significantly
affected in terms of lycopene, sugars contents and pH compared to the
untreated ones, but a tendency to lower content ascorbic acid in the 1-MCP treated
fruits was observed. It was also found a tendency to higher titratable acidity
for 1-MCP treated fruit.
Key words: biological value, tomato, shelf life, storage, 1-MCP .
INTRODUCTION
Tomatoes (Lycopersicon esculentum Mill.) are very important vegetable in human nutrition as they contain a lot of bioactive compounds [17]. Fresh and processed tomatoes are considered as the basic vegetable, suitable to protect human from cancer diseases, especially from prostate cancer [9, 15] and heart diseases [1, 2, 31]. Carotenoid, including lycopene, and phenolic compounds belong to the major contributors to antioxidant activity of fruit and vegetables. Toor and Savage [33] reported that phenolics in tomatoes contribute by about 55% to the antioxidant activity.
There is a need to increase storage ability of vegetables, which is useful for long-distance transport or to prolong ‘shelf life’ of the produce. Therefore, various methods for postharvest treatment of the crops, including 1-MCP treatment, were introduced to horticultural practice. 1-MCP (1-methylcyclopropene) blocks ethylene receptors in cells and prevents ethylene effects in vegetable plant tissues [3, 24, 35]. Tomato fruit is classified as medium-sensitive to ethylene [21]. According to several authors, different factors, including cultivar, ripening stage and storage temperature significantly influence postharvest life and nutritional and sensory quality of tomatoes, as well as the dose and duration of 1-MCP treatment [13, 23, 25, 27, 37]. Tomato fruit treated with 1-MCP shows low respiration rate, ethylene production and weight loss, slower lycopene accumulation and external colour development and prolonged postharvest life than untreated fruit [3, 26, 34, 36].
Tomato fruit obtained from F1 hybrid cultivars with a gene determining long-shelf life (LSL) is characterized by high firmness, what is an important trait for transport and storage [8]. These cultivars differ also from the traditional ones in respect of other important quality attributes, such as colour, texture, taste and nutritional quality [14]. Fresh tomatoes with LSL gene are often harvested at green stage to minimize handling injuries and to maximize postharvest life. However, when the fruit is harvested at near full-ripe stage, it shows better flavour and higher content of sugars and organic acids. There is shortage of information on the influence of 1-MCP treatment on phytochemicals in LSL tomato fruit. As we had found in our previous, initial studies [37, 38], this method could be useful to extend even more shelf-life of the fruit, similarly as it was introduced into practice in the case of ‘traditional’ tomato cultivars and several other species of fruits and vegetables.
The objective of the study was to investigate the influence of 1-MCP treatment at the concentration 1.0 µL·L-1 on some quality traits of tomato fruit ‘Habana’ F1, the cultivar with LSL gene, harvested at mature-green stage and stored at the two temperature variants (12.5°C and 20°C). The additional aim was and to determine differences in the 1-MCP effect on tomato fruit obtained in two growing seasons. Therefore, the study was carried out in two years.
MATERIAL AND METHODS
Plant material
The plant material was purchased from
a commercial farm in central Poland. The storage experiment was conducted in
two growing seasons in the Institute of Horticulture in Skierniewice. The cultivar
used in the study was ‘Habana’ F1,
with LSL gene (Western Seeds, Ltd., Holland; TmVF2N). The plants were grown on
stakes in open field. Standard practices were applied during the cultivation.
The fruit of the cultivar is of medium size (160–175 g weight). The fruit
was harvested in the mature-green (MG) stage of maturity, which is defined by
internal fruit structure indices (fruits are full-sized, seeds are fully developed
and are not cut upon slicing the fruit). Immediately after harvest, chemical
composition of the fruit in the first and the second season was as follows: lycopene
content – 0.2 mg·100 g-1 f.w., ascorbic acid varied from 8.5 to
20.9 mg·100 g-1 f.w., total phenolics – 19.4–21.4 mg·100
g-1 f.w., total sugars – 2.7–2.9% f.w., titratable acidity – 0.5%
and acidity (pH) – 4.4. Average sample weight was 80 kg. Tomatoes without
defects, of the same size and without a calyx were selected for the experiment.
All analysis and measurement were done on representative samples, taken in 4
replicates consisting of 20 fruit in each replicate. The tomatoes were stored
in plastic boxes and covered in the boxes with perforated polyethylene film (PE).
1-MCP was applied directly after the fruit harvest as SmartFreshTM powder, containing
0.14% of active ingredient. The SmartFreshTM powder was placed into 500 mL Erlenmeyer
flasks and afterwards distilled water was added to release 1-MCP in a gas form.
The flask was then placed into 1 m3 volume sealed container. Tomato fruit was
treated with 1.0 μL·L-1 of 1-MCP for 21 h at the temperature of
20ºC and 85–90% RH. Control tomatoes were kept under similar conditions,
but without 1-MCP treatment. After the treatment, all fruits were stored for
4 weeks at the temperature of 12.5ºC (recommended for prolonged storage
of tomatoes) or 20ºC (simulated retail conditions), 85–90% RH, in
ambient atmosphere. After the storage, the fruits were analyzed for lycopene,
ascorbic acid, total phenolics and sugars content, as well as pH and titratable
acidity. Chemical analyses were conducted with 3 replications consist of 10 fruits
in each replications from the fresh plant material and after 4 weeks of storage.
Analytical methods
Lycopene
5 g samples of tomatoes were blended for 5 minutes with 25 ml
mixture of hexane-acetone (4:1 v/v), using Ultra-Turrax T-25 blender. The homogenate
was centrifuged at 5000 G and organic fraction was collected. The residue was
further extracted two times with 5 ml of hexane-acetone mixture until it was
free of red pigment, indicating that lycopene had been completely extracted.
All hexane acetone fractions were combined and washed several times with water
until acetone was completely removed. Lycopene was separated from β-carotene by column chromatography
on aluminum oxide, using method of Czapski and Saniewski [10]. The eluted lycopene
fraction was evaporated to dryness in the dark at room temperature under stream
of nitrogen and re-dissolved in appropriate volume of hexane for spectral measurement
at 472 nm on UviLine 9400 spectrophotometer. The results were expressed in mg·100
g-1 FW.
Ascorbic acid
Ascorbic acid was determined according
to Polish Standard [29]. 20 g samples of tomatoes were homogenized with 200 ml
of 2% oxalic acid. The solution was then filtered. The filtrate was collected
and then titrated with the 2.6-dichlorphenylindophenol. Ascorbic acid content
was calculated from the standard curve and the results were expressed in mg·100
g-1 FW.
Total phenolics
10 g samples of tomatoes were homogenized
with 60 ml of 50% aqueous methanol for 10 min using Ultra Turrax T-25 blender.
The homogenate was then filtered thorough No. 2 Whatman paper on Büchner funnel under vacuum. The residue
was re-extracted with 50% methanol and supernatants were pooled and transferred
to 100 ml volumetric flask and filled to the mark with water. The extract was
analyzed for total phenolics content. Total phenolics content was determined
with Folin-Ciocalteau reagent, according to method used by Emmons et al. [11]
and catechin was used as the standard. Results were expressed in mg·100
g-1 FW.
Total sugars
Total sugars content was determined with
the standard Luff-Schoorl’s
method. The data was expressed as % of FW.
Titratable acidity
Titratable acidity of the fruit was determined with titration method. Homogenized
fruit pulp was titrated with 0.1 M NaOH. The data was expressed as % of citric
acid in FW.
Acidity (pH)
pH value for the fruit pulp was
determined with the potentiometric method, using the potentiometer equipped with
two electrodes.
Statistical analysis
Results of the experiments were statistically evaluated
with ANOVA (Statistica v.10 software), using two-factorial analysis. Tukey’s
HSD test was then used to show which values differ significantly at P=0.05. Due
to significant differences between seasons of the study, the results are discussed
for each season separately.
RESULTS AND DISCUSSION
Lycopene
In the case of lycopene content in tomato,
there was significant influence of storage temperature – in the case of
the higher temperature lycopene content was higher in two storage seasons. On
the base of means of temperature variants 1-MCP treatment negatively affected
lycopene content in the first storage season in the case of storage at the temperature
of 20°C (Tab. 1). However, in the second season, the effect was different, since
the content was lower at the temperature of 12.5°C. So, there was noticed different
relationship for this compound content for the two temperature variants. The
reason for this phenomenon is unknown, but it could be caused by the fruit quality
variation between growing seasons. The highest content of lycopene was found
for 1-MCP treated fruit, stored at 20°C, in the second season, and the lowest
for 1-MCP treated fruits, stored at 12.5°C, in the same season. According to
other authors, the environmental conditions are important factors influencing
tomato plant growth and also quality of the fruit [7, 20]. There are reports
that lycopene content in tomato fruit depends on fruit ripeness, cultivar [4,
22, 39] and storage temperature [19, 30]. Lycopene accumulation and chlorophyll
degradation can be delayed by 1-MCP treatment of the fruit in different stages
of maturity [18]. Ethylene can promote lycopene synthesis, so as to accelerate
lycopene accumulation, therefore inhibition of ethylene production by 1-MCP treatment
could be the reason of decreasing lycopene content after storage of 1-MCP treated
fruit [16, 34].
Table 1. Effect of 1-MCP treatment and storage temperature on the content
of lycopene and ascorbic acid in ‘Habana’ F1 tomatoes, harvested
in mature-green stage, after 4-week storage in two growing seasons |
temperature [°C] (A) |
[mg·100 g-1] |
[mg·100 g-1] |
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Note
to Tab. 1–3: Means in columns and rows for each season marked with
different capital letters differ significantly, according Tukey’s HSD test
at P=0.05. Small letters within the table for each season show if the values
differ significantly for AxB interaction, according to Tukey’s HSD test
at P=0.05. |
Ascorbic acid
The content of ascorbic acid (AA) depended
significantly on storage temperature, but in the second season only (Tab. 1).
The treatment with 1-MCP had significant influence on AA content in the first
storage season. In the second season, the effect of 1-MCP was noticed in the
lower storage temperature only. In the first season, higher content of AA was
found for 1-MCP treated tomatoes. The differences between storage seasons in
AA content after storage may result from different initial AA content in both
years of the experiment. Overall, the lowest AA content was observed for untreated
tomatoes stored at the temperature of 12.5°C (5.6
mg·100 g-1 FW) in the first season. The highest content of AA was found
in treated and untreated tomatoes stored at 20°C (15.9 mg·100 g-1
FW) in the second season. Žnidarčič and Požrl [40] showed
that total reduction of initial AA content was 3.5 mg·100 g-1 (at 5°C)
and 2.5 mg·100 g-1 (at 10°C). According to Wang et al. [34] ascorbic
acid content in 1-MCP treated fruit was always markedly higher than in control
fruit after 8 days of storage. Other authors also observed influence of 1-MCP
treatment on ascorbic acid content in fresh tomatoes [27, 32] and tomato juice
[6].
Titratable acidity
In the
first season, titratable acidity was higher for 1-MCP treated fruit, in both
temperature variants (Tab. 2). In the second season, it was true for the
lower temperature variant only. Mir et al. [25] did not find significant influence
of 1-MCP treatment on titratable acidity of tomato fruit. Temperature of storage
affected titratable acidity for the second season only. In that case the acidity
was higher for temperature of 12.5°C than of 20°C. The effect of temperature
is in agreement with reports of other authors [12, 31]. According to Toor and
Savage [33] high acidity level relates to high vitamin C content in tomatoes.
We did not found such relationship in the study.
Table 2. Effect of 1-MCP treatment and storage temperature on acidity (pH)
and titratable acidity of ‘Habana’ F1 tomatoes harvested in mature-green
stage, after 4-week storage in two growing seasons |
temperature [°C] (A) |
[%] |
[pH] |
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Acidity (pH)
1-MCP treatment
generally did not influence pH value of the tomato fruit (Tab. 2). This is in
agreement with Castro et al. [5] and Mir et al. [25] results. We found small
effect of storage temperature on pH value for the fruit. Higher pH value was
noted for the fruit stored at the temperature of 20°C in the second season. According
to [40], storage temperature had no effect on pH value for tomatoes.
Sugars
1-MCP treatment did not affect sugars content in tomato fruit in both seasons
(Tab. 3). The effect of storage temperature was also insignificant. According
to Mir et al. [25] and Phasey et al. [28] sugars content in tomato fruit treated
with 1-MCP was lower than in control fruit.
Table 3. Effect of 1-MCP treatment and storage temperature on the content
of sugars and phenolics in ‘Habana’ F1 tomatoes harvested in mature-green
stage, after 4-week storage in two growing seasons |
temperature [°C] (A) |
[% f.w.] |
[mg·100 g-1] |
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Phenolics
The effect of 1-MCP treatment on phenolics content was different in
the seasons and also related to storage temperature (Tab. 3). The content was
significantly higher for 1-MCP treated fruits in the case of the first storage
season. The effect of 1-MCP was not observed in the second season. In the second
season, higher phenolics content was observed in the case of the lowers storage
temperature. Wang et al. [34] reported that total phenolics content in 1-MCP
treated tomato fruit was 13% higher than that in the control fruit after storage
for 12 days. Toor and Savage [33] found out that the content of phenolics in
tomatoes increased during storage, regardless of storage temperature. It was
probably due to the disruption of vacuoles as a result of chilling injury that
may have led to loss of some phenolic compounds.
Means of the storage seasons
In Table 4
means of the two storage seasons for all determined quality traits of the tomato
fruit are shown. It can be seen that storage temperature influenced significantly
chemical content of the fruit in the case of most components (with the exception
of sugars). The influence of 1-MCP was more complicated and temperature dependent.
These effects related also to the year of study. Since the tomato fruit showed
different quality changes during storage period in each of the years of the experiment,
it is difficult to draw general conclusions on 1-MCP effect on quality of the
fruit. The reason why the tomatoes differed in this respect may be related to
growing conditions in the field in each season. Therefore, the effect of 1-MCP
on quality of LSL tomato fruit from field production needs further studies.
Table 4. Effect of 1-MCP treatment and storage temperature on quality traits
of ‘Habana’ F1 tomatoes harvested in mature-green stage, after 4-week
storage (means of the two growing seasons) |
temperature [°C] (A) |
![]() |
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[mg·100 g-1] |
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[mg·100 g-1] |
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[%] |
||||
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[pH] |
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[% f.w.] |
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[mg·100 g-1] |
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Note
to Tab. 4: Means in columns and rows for each season marked with different capital
letters differ significantly, according Tukey’s HSD test at P=0.05. |
CONCLUSIONS
Results of the study showed big differentiation in chemical composition of the LSL tomato fruit depending on 1-MCP treatment and storage temperature, but also differences between growing seasons. The relationship between experimental factors studied was not clear and differed between the years. Therefore, it can be concluded in general that the effect of 1-MCP treatment strongly depends on initial physiological state of tomato fruit, which is modified by environmental conditions during growing season. After the storage, a tendency to higher content of phenolics for the fruit treated with 1-MCP was observed. Tomatoes treated with 1-MCP were not significantly affected in terms of lycopene and sugars contents compared to the untreated ones, but a tendency to higher content ascorbic acid in the 1-MCP treated fruits was observed. It was also found a tendency to higher titratable acidity for 1-MCP treated fruit. Results obtained in the study indicate that 1-MCP treatment influenced bioactive compounds changes in stored LSL tomato fruit compared to the untreated fruit, but the relationship between the treatment and the fruit quality parameters was not fully clear.
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Accepted for print: 23.11.2017
Anna Wrzodak
Institute of Horticulture, Poland
Konstytucji 3 Maja 1/3
96-100 Skierniewice
Poland
Justyna Szwejda-Grzybowska
Institute of Horticulture, Poland
Konstytucji 3 Maja 1/3
96-100 Skierniewice
Poland
Marek Gajewski
Warsaw University of Life Sciences – SGGW, Department of Vegetable and Medicinal Plants, Poland
Nowoursynowska 166
02-787 Warszawa
Poland
email: marek_gajewski@sggw.pl
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