Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2017
Volume 20
Issue 4
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Rybus-Zaj±c M. , Korbas A. , Kubi¶ J. 2017. THE EFFECT OF DROUGHT STRESS AND EXCESSIVE UV-B RADIATION ON THE MEMBRANE STATUS IN CUCUMBER COTYLEDONS AND LEAVES, EJPAU 20(4), #07.
Available Online: http://www.ejpau.media.pl/volume20/issue4/abs-07.html

THE EFFECT OF DROUGHT STRESS AND EXCESSIVE UV-B RADIATION ON THE MEMBRANE STATUS IN CUCUMBER COTYLEDONS AND LEAVES

Magdalena Rybus-Zaj±c, Agata Korbas, Jan Kubi¶
Department of Plant Physiology, Poznań University of Life Sciences, Poland

 

ABSTRACT

Crops grown in open ground are from early stages exposed to several stress factors acting simultaneously or in a sequence. One-week and three-week-old seedlings of cucumber plants (Cucumis sativus cv. Dar) were subjected to two abiotic stress factors, i.e. water deficit and UV-B radiation applied separately and in combination. The progressive stresses decreased the water content in cotyledons and leaves. The stresses caused membrane damage and an increase in the injury index (ID), which was the highest under drought and successively lower under UV-B/drought combination and UV-B on its own. An increase in lipoxygenase (LOX) activity and malondialdehyde (MDA) content was observed in cucumber seedlings. UV-B and water deficit caused more pronounced effect separately than in combination. Generally, the damage (injury index) observed in leaves was higher than in cotyledons and the increase in LOX activity was also slightly higher in leaves than in cotyledons. There was greater increase in the MDA content in leaves than in cotyledons.


In nature the abiotic stress  factors – water deficit, excessive radiation and heat, often occur simultaneously.  Plants respond to a combination of two different abiotic stresses in a unique manner and their response is different when stresses are applied individually. The research findings suggest that each of the stresses may alleviate the negative effect of the other stress factors. In the cotyledons and leaves of cucumber seedlings the water deficit and UV-B radiation applied together generally alleviated the effect of individual stresses within the cellular membrane, as measurements of the membrane damage, LOX activity and MDA content revealed.

Key words: abiotic stresses, cucumber, lipoxygenase, malondialdehyde, membrane damage.


Magdalena Rybus-Zaj±c
Department of Plant Physiology, Poznań University of Life Sciences, Poland
Wołyńska 35
60-637 Poznań
Poland
email: magrybus@up.poznan.pl

Agata Korbas
Department of Plant Physiology, Poznań University of Life Sciences, Poland
Wołyńska 35
60-637 Poznań
Poland
email: akorbas@up.poznan.pl

Jan Kubi¶
Department of Plant Physiology, Poznań University of Life Sciences, Poland
Wołyńska 35
60-637 Poznań
Poland
email: jkubis@up.poznan.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.