Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2016
Volume 19
Issue 4
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Oksińska M. , Magnucka E. , Pietr S. 2016. FAST MOLECULAR DETECTION OF PECTOBACTERIUM ATROSEPTICUM, PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM, AND DICKEYA SPP. FROM POTATO (SOLANUM TUBEROSUM L.) STEM TISSUES, EJPAU 19(4), #09.
Available Online: http://www.ejpau.media.pl/volume19/issue4/abs-09.html

FAST MOLECULAR DETECTION OF PECTOBACTERIUM ATROSEPTICUM, PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM, AND DICKEYA SPP. FROM POTATO (SOLANUM TUBEROSUM L.) STEM TISSUES

Małgorzata P. Oksińska, Elżbieta G. Magnucka, Stanisław J. Pietr
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland

 

ABSTRACT

Detection of the pathogenic bacteria causing blackleg of potato plants is important for seed producers, growers and potato industry. The conventional PCR method using primers specific for Pectobacterium atrosepticum (Y45/Y46), Pectobacterium carotovorum subsp. carotovorum (together with Pectobacterium wasabiae) (INPCCF/INPCCR) and Dickeya spp. (Ech1/Ech1’) turned out to be useful to determine the presence of these bacteria among the various microorganisms inhabiting potato stems. In order to minimize the time and costs of a commercial analysis, the pathogen detection method omitted bacterial DNA extraction step. Moreover, universal organic medium was applied for enrichment of the microbial cell number to detection level instead of commonly used isolation of pure bacterial cultures on selective media. Results of our studies confirmed the rising problem of the infection with Dickeya spp. and P. carotovorum subsp. carotovorum which caused the potato plant wilting during hot summer.

Key words: PCR detection, specific primers, potato plant wilting, pectinolytic Enterobacteriaceae.


Małgorzata P. Oksińska
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
phone/fax: +48 71 320 5621
Grunwaldzka 53
50-375 Wrocław
Poland
email: malgorzata.oksinska@up.wroc.pl

Elżbieta G. Magnucka
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
Grunwaldzka 53
50-375 Wrocław
Poland
email: elzbieta.magnucka@up.wroc.pl

Stanisław J. Pietr
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
phone/fax: +48 71 320 6521
Grunwaldzka 53
50-375 Wrocław
Poland
email: stanislaw.pietr@up.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.