Volume 19
Issue 1
Veterinary Medicine
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume19/issue1/abs-10.html
ANTICANCER AND ANTIOXIDANT EFFECTS OF FRUCTOOLIGOSACCHARIDE (FOS) ON CHEMICALLY-INDUCED COLON CANCER IN RATS
Sorial Adly Moharib
Biochemistry Department, National Research Center, Dokki, Cairo, Egypt
ABSTRACT
Onion (Cynara scolymus) and globe artichoke (Allium cepa) fruitswere used as natural sources for isolation of fructooligosaccharides (FOS). Chemical analyses of onion and globe artichoke dry matter revealed the major component was carbohydrates (91.40 and 85.82% respectively). FOS from Onion (FOS1) andglobe artichoke(FOS2) were isolated (45.70 and 42.91% of total carbohydrates respectively). Quantitative and qualitative analysis revealed the presence of kestose, nystose, and fructofuranosyl nystose as major components of FOS1 or FOS2. Cytotoxic activities of FOS1 and FOS2 were examined in vitro using colon (HCT 116), liver (HEPG2), cervical (HELA) and breast (MCF7) carcinoma cell lines. FOS1 or FOS2 showed a higher percentage cell death of HCT-116 carcinoma cell lines than the other cell lines, indicating promising anti-tumorigenic properties, and demonstrating their direct effect on colon cancer cell proliferation. A marked reduction was observed in the levels of ALP (62 and 61%), ALT (70 and 64%) and γ-GT (73 and 65%) in sera of rat groups that first received FOS1 or FOS2 respectively, compared to 1,2 dimethyl hydrazine (DMH) control rats, indicating protective effect of both FOSs. Insignificant change was observed in AST level. Significant decreases in the levels of lipid peroxidation (LP) in sera of rats administered with FOS1 or FOS2 compared to those DMH control rats. Highest significant decrease in the level of LP was observed in sera of rats FOS2 administered more than those of FOS1.The anticancer activity was evaluated through determination of CEA and C19.9 in DMH-induced colon cancer rat groups treated with either FOS1or FOS2 compared with the carcinogenic control rat group (DMH). Therefore, FOS1or FOS2 is more effective for inhibiting DMH-induced colon cancer. The present results showed the activity of GSH-R was increased in liver (56 and 51%) and kidney (54 and 34%) of rat groups treated with FOS1or FOS2, respectively, compared to those of DMH control rats. GSH-P activity was also increased in liver (61 and 58%) and kidney (52 and 51%). FOS1or FOS2 did not modify GSH-T activity in rat groups. FOS1or FOS2 exhibited a higher increase of SOD activity in liver (67 and 61%) and kidney (59 and 54%), respectively, as compared to those of DMH control rats. The most significant findings of the present study is that the FOS1 or FOS2 (200 mg/kg body weight) have shown a beneficial effect not only on colon cancer but also on antioxidant enzymes activity in DMH - induced colon cancer in rats, as well as protected the cell against DMH oxidative stress by antagonizing DMH toxicity. According to these observations, the use of FOS (FOS1or FOS2) can be recommended as anticancer and antioxidant agents.
Key words: FOS, Onion, Artichoke, Anticancer, Antioxidant, Rat.
Sorial Adly Moharib
Biochemistry Department, National Research Center, Dokki, Cairo, Egypt
email: smoharib@yahoo.com
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.