Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2016
Volume 19
Issue 1
Topic:
Biology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Mahmoodzadeh H. , Fatehi H. 2016. IN VITRO REGENERATION OF VALERIAN (VALERIANA OFFICINALIS L.) INDUCED BY DIFFERENT CONCENTRATIONS OF NAA AND BAP, EJPAU 19(1), #02.
Available Online: http://www.ejpau.media.pl/volume19/issue1/abs-02.html

IN VITRO REGENERATION OF VALERIAN (VALERIANA OFFICINALIS L.) INDUCED BY DIFFERENT CONCENTRATIONS OF NAA AND BAP

Homa Mahmoodzadeh, Hida Fatehi
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

 

ABSTRACT

In this research, regeneration of Valeriana officinalis via
shoot apex was studied. Shoot apex explants were cultured on MS medium supplemented
with different concentrations and combinations of NAA and BAP. The highest callus
induction percentage was obtained in the medium containing 0.5 mg/l BAP and 0.5
mg/l NAA. Multiple shoot were induced from the shoot apex explant of V. officinalis by
culturing them in MS medium supplemented with 0.5 mg/l of 6-benzylaminopurine
(BAP). The explants also induced root formation in MS medium supplemented with
1 mg/l of NAA. The highest fresh and dry weight of callus was observed when 0.5
mg/l BAP was combined with 0.5 mg/l NAA. The addition of BAP up to 1 mg/l to
the medium containing different concentrations of NAA lead to the production
of callus which is compact rather than friable. This result suggests that this
methodology can be applied for rapid propagation of this species.

Key words: Propagation, MS medium, Explant, Culture.


Homa Mahmoodzadeh
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

email: homa_mahmoodzadeh@yahoo.com

Hida Fatehi
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.