Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2015
Volume 18
Issue 4
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Tomaszewska-Hetman L. , Rywińska A. 2015. ERYTHROSE REDUCTASE AS AN ENZYME RESPONSIBLE FOR ERYTHRITOL OVERPRODUCTION IN YARROWIA LIPOLYTICA YEAST GROWN ON GLYCEROL MEDIA, EJPAU 18(4), #08.
Available Online: http://www.ejpau.media.pl/volume18/issue4/abs-08.html

ERYTHROSE REDUCTASE AS AN ENZYME RESPONSIBLE FOR ERYTHRITOL OVERPRODUCTION IN YARROWIA LIPOLYTICA YEAST GROWN ON GLYCEROL MEDIA

Ludwika Tomaszewska-Hetman, Anita Rywińska
Department of Biotechnology and Food Microbiology, Wroc³aw University of Environmental and Life Sciences, Poland

 

ABSTRACT

On the basis of initial screening of Yarrowia lipolytica strains,
performed in the shake-flasks experiment using a glycerol medium, three strains
with different ability of erythritol production were selected. The yield and
dynamics of the biosynthesis by chosen strains were examined in bioreactor cultures
and the activities of enzymes responsible for glycerol consumption and erythritol
synthesis were analyzed during the process. The comparison of  relationship
between erythritol production ability and activities of the examined enzymes
indicated erythrose reductase (ER) as the key enzyme responsible for erythritol
overproduction in Y. lipolytica cells grown on glycerol. In the culture,
the activity of ER in the effective phase of erythritol biosynthesis reached
0.068–0.248 U mg-1, whereas erythritol production was from 44.7 to 75.9
g L-1 with the yield of 0.31–0.51 g g-1. The cells ability of erythritol
formation depended slightly on the glycerol kinase and glycerol-3-phosphate dehydrogenase.

Key words: erythritol, glycerol, Yarrowia lipolytica, enzyme activity.

Ludwika Tomaszewska-Hetman
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Phone: +48 713207793
Fax: +48 713207794
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: Ludwika.Tomaszewska@up.wroc.pl

Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: anita.rywinska@wnoz.up.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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