EJPAU 2015. Andrzejewska J. , Ignaczak S. , Katańska-Kaczmarek A. , Barzyk P. CRUDE FAT CONTENT AND FATTY ACID PROFILE IN THE SEEDS OF POLISH LINES AND CULTIVARS OF FIELD PEA (<i>PISUM SATIVUM</i> L.): PILOT STUDY

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2015
Volume 18
Issue 4

Topic:

Agronomy

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Andrzejewska J. , Ignaczak S. , Katańska-Kaczmarek A. , Barzyk P. 2015. CRUDE FAT CONTENT AND FATTY ACID PROFILE IN THE SEEDS OF POLISH LINES AND CULTIVARS OF FIELD PEA (PISUM SATIVUM L.): PILOT STUDY, EJPAU 18(4), #07.
Available Online: http://www.ejpau.media.pl/volume18/issue4/abs-07.html

CRUDE FAT CONTENT AND FATTY ACID PROFILE IN THE SEEDS OF POLISH LINES AND CULTIVARS OF FIELD PEA (PISUM SATIVUM L.): PILOT STUDY

Jadwiga Andrzejewska¹, Stanisław Ignaczak¹, Agnieszka Katańska-Kaczmarek², Paweł Barzyk³
¹ Department of Agrotechnology, University of Science and Technology in Bydgoszcz, Poland
² Danko Plant Breeders LTD, Szelejewo Department, Poland
³ Poznańska Hodowla Roślin LTD, Plant Breeding Station Wiatrowo, Poland

ABSTRACT

The objective of the presented study was to induced mutation in two liatris
species L. ligulistylis and L. pycnostachya using ethyl methane-sulfonate
(EMS) at a concentration of 2.0 and 5.0 mM and characterization of the obtained
mutants. The resulting phenotypic changes observed in liatris were mainly related
to anthocyanin discoloration on shoots and inflorescence petioles, changes in
the anatomy of inflorescence petioles and leaves, as well as the uneven rising
of inflorescence buds. The EMS mutagen concentrations used in the experiment
revealed an inhibitory effect on features such as the height and width of plants,
the circumference and weight of bulbs and the number of buds on the bulb. It
was found that, the frequency of all observed phenotypic changes in L. ligulistylis and L.
pycnostachya was higher after the application of 2.0 mM EMS solution (11
and 3%, respectively).

For the assessment of genotypic changes at the DNA level the inter simple
sequence repeat (ISSR) markers were adopted. Based on the dendrogram produced
from a study analysis of the similarity coefficients using UPGMA it was found
that the mutants under study showed a 43.5–75.7% similarity to each other.

Key words: colourful blossom pea, white blossom pea, alpha-linolenic acid, linolic acid, oleic acid.

Jadwiga Andrzejewska
Department of Agrotechnology, University of Science and Technology in Bydgoszcz, Poland
Kordeckiego 20
85-225 Bydgoszcz
Poland
email: jadwiga@utp.edu.pl

Stanisław Ignaczak
Department of Agrotechnology, University of Science and Technology in Bydgoszcz, Poland
Kordeckiego 20
85-225 Bydgoszcz
Poland

Agnieszka Katańska-Kaczmarek
Danko Plant Breeders LTD, Szelejewo Department, Poland
Szelejewo Drugie 5
63-820 Piaski
Poland

Paweł Barzyk
Poznańska Hodowla Roślin LTD, Plant Breeding Station Wiatrowo, Poland
Wiatrowo 16,
62-100 Wągrowiec
Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.