Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2015
Volume 18
Issue 4
Topic:
Horticulture
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Krupa-Małkiewicz M. , Wraga K. , Kantarek Z. 2015. INDUCED CHEMICAL MUTATION IN LIATRIS LIGULISTYLIS AND LIATRIS PYCNOSTACHYA, EJPAU 18(4), #06.
Available Online: http://www.ejpau.media.pl/volume18/issue4/abs-06.html

INDUCED CHEMICAL MUTATION IN LIATRIS LIGULISTYLIS AND LIATRIS PYCNOSTACHYA

Marcelina Krupa-Małkiewicz1, Krzysztof Wraga2, Zuzanna Kantarek1
1 Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Poland
2 Study of Ornamental Plants, West Pomeranian University of Technology in Szczecin, Poland

 

ABSTRACT

The objective of the presented study was to induced mutation in two liatris
species L. ligulistylis and L. pycnostachya using ethyl methane-sulfonate
(EMS) at a concentration of 2.0 and 5.0 mM and characterization of the obtained
mutants. The resulting phenotypic changes observed in liatris were mainly related
to anthocyanin discoloration on shoots and inflorescence petioles, changes in
the anatomy of inflorescence petioles and leaves, as well as the uneven rising
of inflorescence buds. The EMS mutagen concentrations used in the experiment
revealed an inhibitory effect on features such as the height and width of plants,
the circumference and weight of bulbs and the number of buds on the bulb. It
was found that, the frequency of all observed phenotypic changes in L. ligulistylis and L.
pycnostachya
was higher after the application of 2.0 mM EMS solution (11
and 3%, respectively).


For the assessment of genotypic changes at the DNA level the inter simple
sequence repeat (ISSR) markers were adopted. Based on the dendrogram produced
from a study analysis of the similarity coefficients using UPGMA it was found
that the mutants under study showed a 43.5–75.7% similarity to each other.

Key words: mutants, DNA, EMS, ISSR analyssis, liatria.


Marcelina Krupa-Małkiewicz
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Poland
Słowackiego St. 17,
71-434 Szczecin, Poland
email: mkrupa@zut.edu.pl

Krzysztof Wraga
Study of Ornamental Plants, West Pomeranian University of Technology in Szczecin, Poland
3A Papieża Pawła VI St.
71-459 Szczecin, Poland

Zuzanna Kantarek
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Poland
Słowackiego St. 17,
71-434 Szczecin, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.