Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2015
Volume 18
Issue 4
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Piegza M. , Jurkiewicz M. , Kancelista A. , £aba W. , Witkowska D. 2015. MOULDS LYTIC ENZYMES IN THE PRODUCTION OF YARROWIA HIGH-PROTEIN FEED ADDITIVES, EJPAU 18(4), #04.
Available Online: http://www.ejpau.media.pl/volume18/issue4/abs-04.html

MOULDS LYTIC ENZYMES IN THE PRODUCTION OF YARROWIA HIGH-PROTEIN FEED ADDITIVES

Micha³ Piegza, Marcin Jurkiewicz, Anna Kancelista, Wojciech £aba, Danuta Witkowska
Department of Biotechnology and Food Microbiology, Wroc³aw University of Environmental and Life Sciences, Poland

 

ABSTRACT

The optimal conditions for the degradation of the cell wall of Yarrowia lipolytica yeast, leading to the release of intracellular proteins by hydrolases of Trichoderma and Aspergillus moulds, were determinated. Hydrolysis of Y. lipolytica yeast biomass was performed with the use of various amounts of concentrated or non-concentrated enzymes, as well as yeast biomass subjected or not subjected to thermal inactivation. The analysis of enzymatic hydrolysis process demonstrated that the most effective was the 8-hour hydrolysis of thermally not inactivated Y. lipolytica yeast biomass with 6-fold concentrated enzymatic preparation, which resulted in the release of 127.86 mg of intracellular proteins per 1 g dry biomass.

Key words: chitinases, SCP, Trichoderma, Aspergillus, laminarinases.


Micha³ Piegza
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: mikey@wnoz.ar.wroc.pl

Marcin Jurkiewicz
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland

Anna Kancelista
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland

Wojciech £aba
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland

Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 37/41
51-630 Wroc³aw
Poland
email: wit@ozi.ar.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.