Volume 18
Issue 1
Agronomy
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume18/issue1/abs-02.html
PLANT GROWTH REGULATORS AFFECTING IN VITRO CALLUS, SHOOTS AND ROOTS INDUCTION OF SILPHIUM PERFOLIATUM L. – A NEW ALTERNATIVE MULTIFUNCTIONAL PLANT
Magdalena Tomaszewska-Sowa, Anna Figas
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland
ABSTRACT
The aim of the study was to investigate the optimal factors needed for establishing
an effective protocol for propagation of the cup plant. This species can be applied
in medicine, animal feeding, and as a decorative, apiarian energy-producing and fitoremediation
plant. Currently, there is no sufficient sowing material in Poland that would
do justice to the present attempts at propagating this species in in vitro growth
cultures. As explants were used cotyledonary and leaf fragments with petioles,
which were placed onto MS media supplemented with BAP in combination with IAA,
ABA, NAA; and onto MS medium fortified with KIN and 2,4 – for callus and
shoot bud initiation. The most efficient callus proliferation reported was on
MS medium incorporated with various concentrations and combinations of auxins
and cytokinins such as BAP (10 mg·dm-3) + NAA (1.0 mg·dm-3), BAP (0.5
mg·dm-3) + 2,4-D (1.0 mg·dm-3) + IAA (1.0 mg·dm-3) + NAA (1.0 mg·dm-3).
The adventitious shoots formed from both the tested explants. It was found that
the highest frequency of explants forming shoots were induced on MS medium containing
BAP (5.0 mg·dm-3) + IAA (0.05 mg·dm-3) and KIN (0.2 mg·dm-3) + IAA (2.0
mg·dm-3), respectively.
Key words: Cup plant, micropropagation, callus, explants, cytokinins, auxins, in vitro.
Magdalena Tomaszewska-Sowa
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland
email: magda@utp.edu.pl
Anna Figas
Department of Agricultural Biotechnology, University of Science and Technology, Bydgoszcz, Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.