Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2015
Volume 18
Issue 1
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Tomaszewska-Sowa M. , Figas A. 2015. PLANT GROWTH REGULATORS AFFECTING IN VITRO CALLUS, SHOOTS AND ROOTS INDUCTION of Silphium perfoliatum L. – A NEW ALTERNATIVE MULTIFUNCTIONAL PLANT, EJPAU 18(1), #02.
Available Online: http://www.ejpau.media.pl/volume18/issue1/abs-02.html

PLANT GROWTH REGULATORS AFFECTING IN VITRO CALLUS, SHOOTS AND ROOTS INDUCTION OF SILPHIUM PERFOLIATUM L. – A NEW ALTERNATIVE MULTIFUNCTIONAL PLANT

Magdalena Tomaszewska-Sowa1, Anna Figas2
1 Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland
2 Department of Plant Physiology and Fundamentals of Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences in Bydgoszcz, Poland

 

ABSTRACT

The aim of the study was to investigate the optimal factors needed for establishing
an effective protocol for propagation of the cup plant. This species can be applied
in medicine, animal feeding, and as a decorative, apiarian energy-producing and  fitoremediation
plant. Currently, there is no sufficient sowing material in Poland that would
do justice to the present attempts at propagating this species in in vitro growth
cultures. As explants were used cotyledonary and leaf fragments with petioles,
which were placed onto MS media supplemented with BAP in combination with IAA,
ABA, NAA; and onto MS medium fortified with KIN and 2,4 – for callus and
shoot bud initiation. The most efficient callus proliferation reported was on
MS medium incorporated with various concentrations and combinations of auxins
and cytokinins such as BAP (10 mg·dm-3) + NAA (1.0 mg·dm-3), BAP (0.5
mg·dm-3) + 2,4-D (1.0 mg·dm-3) + IAA (1.0 mg·dm-3) + NAA (1.0 mg·dm-3).
The adventitious shoots formed from both the tested explants. It was found that
the highest frequency of explants forming shoots were induced on MS medium containing
BAP (5.0 mg·dm-3) + IAA (0.05 mg·dm-3) and KIN (0.2 mg·dm-3) + IAA (2.0
mg·dm-3), respectively.

Key words: Cup plant, micropropagation, callus, explants, cytokinins, auxins, in vitro.


Magdalena Tomaszewska-Sowa
Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland
Bernardyńska 6
85-029 Bydgoszcz
Poland
email: magda@utp.edu.pl

Anna Figas
Department of Plant Physiology and Fundamentals of Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6
85-029 Bydgoszcz
Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.