Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2014
Volume 17
Issue 3
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Gębarowska E. , Szumny A. , Pietr S. 2014. APPLICABILITY OF DISTILLATION-EXTRACTION TECHNIQUE FOR ISOLATION OF VOLATILE COMPOUNDS FROM DIFFERENT CULTURES OF MICROSCOPIC FUNGI, EJPAU 17(3), #13.
Available Online: http://www.ejpau.media.pl/volume17/issue3/abs-13.html

APPLICABILITY OF DISTILLATION-EXTRACTION TECHNIQUE FOR ISOLATION OF VOLATILE COMPOUNDS FROM DIFFERENT CULTURES OF MICROSCOPIC FUNGI

Elżbieta Gębarowska1, Antoni Szumny2, Stanisław J. Pietr3
1 Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
2 Department of Chemistry, Wrocław University of Environmental and Life Sciences, Poland
3 Laboratory of Agricultural Microbiology, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Wrocław, Poland

 

ABSTRACT

The aim of the study was to evaluate the effectiveness of this simple, solvent-free, one-step distillation-extraction method for the isolation of volatile metabolites from cultures of microscopic fungi applying a Deryng apparatus. For this study we tested liquid and solid-state cultures of filamentous fungi of Trichoderma viride, T. harzianum and Aspergillus niger and yeast fungi of Rhodotorula glutinis and Saccharomyces cerevisiae. The volatile compound, 6-pentyl-alpha-pyrone, was isolated via steam distillation, applying the Deryng apparatus as the only volatile metabolite from liquid and solid cultures of tested fungal cultures in the range from 2,1 to 109,6 μg in 1 mL or 1 g fungal cultures.

Key words: fungal volatile metabolites, lactones, Deryng apparatus, filamentous fungi, yeast.

Elżbieta Gębarowska
Agricultural Microbiology Lab, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Poland
Grunwaldzka 53
53-636 Wrocław
Poland
email: elzbieta.gebarowska@up.wroc.pl

Antoni Szumny
Department of Chemistry, Wrocław University of Environmental and Life Sciences, Poland
Norwida 25
50-375 Wrocław
Poland
email: antoni.szumny@up.wroc.pl

Stanisław J. Pietr
Laboratory of Agricultural Microbiology, Department of Plant Protection, Wrocław University of Environmental and Life Sciences, Wrocław, Poland
phone/fax: +48 71 320 6521
Grunwaldzka 53
50-375 Wrocław
Poland
email: stanislaw.pietr@upwr.edu.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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