Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2014
Volume 17
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Piegza M. , Szlączka K. , Łaba W. , Witkowska D. 2014. EFFECT OF CARBON SOURCE ON THE PRODUCTION OF LYTIC ENZYMES BY FILAMENTOUS FUNGI OF THE TRICHODERMA GENUS, EJPAU 17(2), #06.
Available Online: http://www.ejpau.media.pl/volume17/issue2/abs-06.html

EFFECT OF CARBON SOURCE ON THE PRODUCTION OF LYTIC ENZYMES BY FILAMENTOUS FUNGI OF THE TRICHODERMA GENUS

Michał Piegza, Karolina Szlączka, Wojciech Łaba, Danuta Witkowska
Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Poland

 

ABSTRACT

The aim of this study was to evaluate the effect of cell wall biopolymers
present in biomass of different types of filamentous fungi, yeasts and in biomass
of insects on the expression of lytic enzymes in selected representatives of
filamentous fungi: Trichoderma harzianum T33 and Trichoderma citrinoviride C1.
Dried biomass of filamentous fungi, yeast and insect cells constituted a carbon
source and also acted as an inductor of evaluated enzymes biosynthesis. The biosynthesis
dynamics of chitinases, proteases and laminarinases depending on the type of
inductor introduced into the culture were characterized and compared to the control
cultures, not complemented with the cell wall biopolymers. The differences in
the kinds and level of enzymatic activity, due to the addition of carbon source
were demonstrated. The addition of insect biomass (1%) was the most preferred
for expression of laminarinases and proteases in both Trichoderma strains
(with a little predominance in the C1 strain). Geotrichum and Botrytis biomass
was most effective in the induction of expressed chitinases (with a little predominance
in T33 strain).

Key words: Trichoderma, chitinases, beta-1,3-glucanases, Musca.


Michał Piegza
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: mikey@wnoz.ar.wroc.pl

Karolina Szlączka
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland


Wojciech Łaba
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland

Danuta Witkowska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: wit@ozi.ar.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.