EJPAU 2013. Packa D. , Graban Ł. , Lajszner W. , Załuski D. , Hościk M. ALPHA-AMYLASE ACTIVITY IN THE KERNELS OF HULLED SPRING WHEAT AFTER HEAD INOCULATION WITH <i>FUSARIUM CULMORUM</i> (W.G.SMITH) SACC.

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2013
Volume 16
Issue 4

Topic:

Agronomy

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Packa D. , Graban Ł. , Lajszner W. , Załuski D. , Hościk M. 2013. ALPHA-AMYLASE ACTIVITY IN THE KERNELS OF HULLED SPRING WHEAT AFTER HEAD INOCULATION WITH FUSARIUM CULMORUM (W.G.SMITH) SACC., EJPAU 16(4), #06.
Available Online: http://www.ejpau.media.pl/volume16/issue4/abs-06.html

ALPHA-AMYLASE ACTIVITY IN THE KERNELS OF HULLED SPRING WHEAT AFTER HEAD INOCULATION WITH FUSARIUM CULMORUM (W.G.SMITH) SACC.

Danuta Packa, Łukasz Graban, Waldemar Lajszner, Dariusz Załuski, Michał Hościk
Department of Plant Breeding and Seed Production, University of Warmia and Mazury, Olsztyn, Poland

ABSTRACT

Alpha-amylase activity was studied in the kernels of four wheat species, including
11 accessions of T. monococcum L., 11 accessions of T. dicoccum (Schrank)Schuebl.,
4 accessions of T. spelta L. and 4 cultivars of T. aestivum L.,
after head inoculation with a spore suspension of Fusarium culmorum. Alpha-amylase
activity was determined using the Ceralpha Method. This method is recommended
for measuring the activity of alpha-amylase of both plant and microbial origin.
Kernels from inoculated treatments showed increased levels of alpha-amylase activity
in all tested species except for T. spelta in 2008. T. aestivum was
characterized by the lowest alpha-amylase activity, both in non-inoculated (control)
and inoculated treatments.

Key words: Ceralpha Method, hulled wheats, Triticum spp.

Danuta Packa
Department of Plant Breeding and Seed Production,
University of Warmia and Mazury, Olsztyn, Poland
tel. 89 524 53 29
pl. Łodzki 3, 10-724 Olsztyn, Poland
email: packa@uwm.edu.pl

Łukasz Graban
Department of Plant Breeding and Seed Production,
University of Warmia and Mazury, Olsztyn, Poland
pl. Łodzki 3, 10-724 Olsztyn, Poland

Waldemar Lajszner
Department of Plant Breeding and Seed Production,
University of Warmia and Mazury, Olsztyn, Poland
pl. Łodzki 3, 10-724 Olsztyn, Poland

Dariusz Załuski
Department of Plant Breeding and Seed Production,
University of Warmia and Mazury, Olsztyn, Poland
pl. Łodzki 3, 10-724 Olsztyn, Poland
email: dariusz.zaluski@uwm.edu.pl

Michał Hościk
Department of Plant Breeding and Seed Production,
University of Warmia and Mazury, Olsztyn, Poland
pl. Łodzki 3, 10-724 Olsztyn, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.