Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

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Prażak R. 2013. THE INFLUENCE OF ZINC ON GROWTH AND DEVELOPMENT OF DENDROBIUM KINGIANUM BIDWILL IN IN VITRO CULTURE, EJPAU 16(2), #06.
Available Online: http://www.ejpau.media.pl/volume16/issue2/abs-06.html

ABSTRACT

The influence of increased zinc content in Murashige and Skoog solid medium
on the growth and development of the orchid Dendrobium kingianum Bidwill
was studied. Sterile explants of pseudobulbs were used for micropropagation of
the orchid plant on MS (1962) regeneration medium supplemented with 0.5 mg dm^-3
NAA and 1.0 mg dm^-3 kinetin. Zinc (as ZnSO4 × 7H2O) was added to all combinations
in concentrations 2, 4, 8 and 16 times bigger (17.2, 34.4, 68.8, 137.6 mg dm^-3)
than the standard content (8.6 mg dm^-3) in MS-medium (control). The obtained
results showed that treatment with 2 times increased zinc concentration stimulated
the plant growth and development. After six and twelve months in in vitro culture
the biggest number of shoots and roots, length of shoots and roots and the biggest
fresh weight of plants were obtained in media with 2 times greater zinc content
than in control (standard content of zinc in MS-medium). However in media with
8 and 16 times bigger zinc content, negative influence of zinc on the biometrical
features was noted.

Key words: orchids, tissue culture, micropropagation, zinc sulphate.

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Roman Prażak
Department of Plant Biology, Faculty of Agricultural Sciences in Zamość, University of Life Sciences in Lublin, Poland
Phone: (+48) 67 72 729
Szczebrzeska 102
22-400 Zamość, Poland
email: roman.prazak@up.lublin.pl

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