Volume 16
Issue 2
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume16/issue2/abs-05.html
EFFECT OF NUTRIENT DEPRIVATION STRESS ON SEEDLINGS MORPHOLOGY AND ROS FORMATION IN SELECTED RILS OF RYE (SECALE CEREALE L.)
Mi這sz Smolik1, Patrycja Cieluch2, Kinga Mazurkiewicz-Zapa這wicz3
1 Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin
2 Division of Hydrobiology, Ichthyology and Biotechnology of Breeding, West Pomeranian University of Technology in Szczecin
3 Division of Hydrobiology, Ichthyology and Biotechnology of Biotechnology of Reproduction,
West Pomeranian University of Technology in Szczecin, Szczecin, Poland
ABSTRACT
The study presents the possibility of localization of reactive oxygen species (ROS) in cytological preparations of seminal roots and coleoptiles of eleven-day-old seedlings of recombinant inbred lines (RILs) of rye, subjected to the influence of abiotic stress caused by nitrogen and potassium deficiencies in a medium in in vitro cultures of mature embryos. Fifty-one recombinant inbred lines of rye (F9) considered as tolerant and susceptible to stress caused by nitrogen and potassium deficiencies in a medium were selected for the research. The plants were derived from the F2 generation, obtained from a combination of crossings of inbred lines of rye Ot0-6 and Ot1-3. The response of each line to stress was assessed under the conditions of high and decreased level of N and K in a medium. In the medium with high N and K content the seedlings developed on average longer coleoptiles, shorter the longest roots and less numerous roots in comparison with the medium with low content of N and K. Other described lines included lines which developed shorter coleoptiles and significantly shorter the longest roots on the medium with deficient nitrogen and potassium in comparison with the medium with a high content of nitrogen and potassium. Depending on individual morphological responses of the seedlings, the lines were clustered with the use of Ward's agglomerative method with regard to the response of three seedling traits: coleoptile length, the longest root length and root number. RILs with extreme responses to nutrient stress were identified in the outermost groups of Ward's dendrogram. ROS , mainly H2O2, were visualized in the epidermal part of the elongation zone of the seminal roots of seedlings subjected to the influence of nutrient stress. More intense fluorescence of the fluorescence dye (2',7'-dichlorofluorescein) was detected in the seedlings of the lines considered as susceptible to nutrient deficiencies as compared to tolerant lines. The generation of ROS , as important signal molecules provesfunctioning of the molecular mechanisms of sensing and plant cells response to nutrient stress.
Key words: rye, nitrogen-potassium starvation, ROS, dichlorofluorescein, inverted microscopy.
Mi這sz Smolik
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin
S這wackiego St. 17,
71-434 Szczecin, Poland
Phone: +48 91 449 61 95
email: msmolik@zut.edu.pl
Patrycja Cieluch
Division of Hydrobiology, Ichthyology and Biotechnology of Breeding, West Pomeranian University of Technology in Szczecin
S這wackiego St. 17,
71-434 Szczecin, Poland
Kinga Mazurkiewicz-Zapa這wicz
Division of Hydrobiology, Ichthyology and Biotechnology of Biotechnology of Reproduction,
West Pomeranian University of Technology in Szczecin, Szczecin, Poland
K. Kr鏊ewicza 4
71-550 Szczecin
Poland
email: Kinga.Mazurkiewicz-zapalowicz@zut.edu.pl
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.