EJPAU 2011. Romanek J. , Walczak H. , Wójcik-Jagła M. , Jurczyk B. , Rapacz M. THE EFFECT OF SIMULATED DROUGHT ON <i>HVA1</i> AND <i>SRG6</i> GENE EXPRESSION IN SPRING BARLEY

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2011
Volume 14
Issue 3

Topic:

Biotechnology

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Romanek J. , Walczak H. , Wójcik-Jagła M. , Jurczyk B. , Rapacz M. 2011. THE EFFECT OF SIMULATED DROUGHT ON HVA1 AND SRG6 GENE EXPRESSION IN SPRING BARLEY, EJPAU 14(3), #04.
Available Online: http://www.ejpau.media.pl/volume14/issue3/abs-04.html

THE EFFECT OF SIMULATED DROUGHT ON HVA1 AND SRG6 GENE EXPRESSION IN SPRING BARLEY

Joanna Romanek¹, Hanna Walczak², Magdalena Wójcik-Jagła², Barbara Jurczyk², Marcin Rapacz²
¹ Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production
² Department of Plant Physiology University of Agriculture in Krakow

ABSTRACT

Drought is one of the most important environmental stresses in agriculture.
It significantly reduces both the quality and yield of agricultural crops. Under
conditions in Poland, spring barley is more sensitive to drought than winter
barley. In plants, the expression of dehydrins increases the ability to
absorb and hold water in the cell. One can try to obtain plants with increased
resistance to drought, among other things, through the selection of genotypes
with increased accumulation of dehydrins.

The aim of our study was to examine
differences in the expression levels of SRG6 and HVA1 genes
in Polish breeding lines of spring barley. In this experiment measurements of
the accumulation levels of HVA1 and SRG6 transcripts by means of
real-time PCR were performed in twenty seven barley genotypes. Estimations of
mRNA abundance were made before drought and on the 15th day of the experiment
at a relative maximum water capacity of 32%. The results of our experiments showed
that drought stress caused a significant increase in the expression of HVA1 and SRG6 genes
in all genotypes. The 27 studied breeding strains of spring barley from Polish
breeding companies were characterized by high variation in the expression of
both genes.

Key words: Drought, barley, HVA1, SRG6, water deficit tolerance.

Joanna Romanek
Department of Biotechnology of Animal Reproduction,
National Research Institute of Animal Production
Krakowska 1,
32-083 Balice
email: jromanek@izoo.krakow.pl

Hanna Walczak
Department of Plant Physiology
University of Agriculture in Krakow
Podłużna 3
30-239 Krakow, Poland

Magdalena Wójcik-Jagła
Department of Plant Physiology
University of Agriculture in Krakow
Podłużna 3
30-239 Krakow, Poland

Barbara Jurczyk
Department of Plant Physiology
University of Agriculture in Krakow
Podłużna 3
30-239 Krakow, Poland

Marcin Rapacz
Department of Plant Physiology
University of Agriculture in Krakow
Podłużna 3
30-239 Krakow, Poland

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