Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Moharib S. , Abdel-Rahman T. , Abdel-Maugod Moussa T. , Yehia R. 2011. EFFECT OF MEDIA COMPOSITION ON LACCASE PRODUCTION BY PLEUROTUS OSTREATUS IN BATCH CULTURE, EJPAU 14(3), #03.
Available Online: http://www.ejpau.media.pl/volume14/issue3/abs-03.html

ABSTRACT

Laccases, enzymes involved in lignin degradation are produced by various organisms, specially fungi. The addition of inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. Eighteen media were prepared using full L18 (2¹ x 3⁷) factorial design to maximize the production of laccase by Pleurotus ostreatus. When wheat bran was used as solid substrate, maximum laccase activity was recorded in medium no. 7, while wheat bran extract was added highest enzyme activity was in medium no. 15. Generally, solid wheat bran substrate induced high laccase activity while, bran extract induced higher protein content in culture filtrates and dry biomass of P.ostreatus in all media. The biochemical parameters (protein and sugars) and growth parameters (mycelial growth rate and dry biomass) varied significantly with media composition and nature of wheat bran added as substrate (solid or extract). Maximum of extracellular laccase activity was observed in medium no.7 together with high amount of protein, reducing (DRS) and non-reducing sugars (NRS). Promising degradability action of P.ostreatus especially in presence of solid bran substrate was observed in this medium.

Key words: laccase, Pleurotus ostreatus, biochemical parameters, growth, biomass.

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Sorial Adly Moharib
Biochemistry Department, National Research Center, Dokki, Cairo, Egypt

email: smoharib@yahoo.com

Tahany Mohammed Abdel-Rahman
Botany Department,
Faculty of Science, Cairo University Egypt


Tarek Abdel-Maugod Moussa
Botany Department,
Faculty of Science, Cairo University Egypt


Ramy Sayed Yehia
Botany Department,
Faculty of Science, Cairo University Egypt

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