EJPAU 2011. Mazurkiewicz J. DEGRADATION OF OCHRATOXIN A BY <I>Lactobacillus acidophilus</I> K1

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2011
Volume 14
Issue 2

Topic:

Biotechnology

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Mazurkiewicz J. 2011. DEGRADATION OF OCHRATOXIN A BY Lactobacillus acidophilus K1, EJPAU 14(2), #16.
Available Online: http://www.ejpau.media.pl/volume14/issue2/abs-16.html

DEGRADATION OF OCHRATOXIN A BY LACTOBACILLUS ACIDOPHILUS K1

Jarosław Mazurkiewicz
Department of Biotechnology, Human Nutrition and Science of Food Commodities, University of Life Sciences in Lublin, Poland

ABSTRACT

Degradation of ochratoxin A (OTA) was performed in 16 and 24-hour long incubation in PBS of various pH (4 to 7). Standard OTA was dissolved in acetonitrile and added to PBS. The concentration of mycotoxin was determined with the use of direct competence ELISA tests. Dead cells of bacteria were obtained by the thermal inactivation in PBS. The dead cells of Lactobacillus acidophilus K1 strain have the highest ability to degrade OTA after 24 hours of incubation in PBS at pH 5. At these conditions OTA was degraded by 79%. Lowering of pH caused an increase in the OTA degradation. After 16 hours of incubation at pH 4 the residue of mycotoxin amounted to 25%. The elongation of incubation time caused lowering of mycotoxin concentration. At the shorter time of incubation, live cells showed higher ability to eliminate OTA.

Key words: Ochratoxin A, degradation, ELISA, Lactobacillus acidophilus.

Jarosław Mazurkiewicz
Department of Biotechnology,
Human Nutrition and Science of Food Commodities,
University of Life Sciences in Lublin, Poland
Skromna 8, 20-704 Lublin, Poland
email: jaroslaw.mazurkiewicz@up.lublin.pl

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