EJPAU 2010. Zalewska M. , Miler N. , Tymoszuk A. , Drzewiecka B. , Winiecki J. RESULTS OF MUTATION BREEDING ACTIVITY ON <I>Chrysanthemum</I> × <I>grandiflorum </I>(Ramat.) Kitam. IN POLAND

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2010
Volume 13
Issue 4

Topic:

Horticulture

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Zalewska M. , Miler N. , Tymoszuk A. , Drzewiecka B. , Winiecki J. 2010. RESULTS OF MUTATION BREEDING ACTIVITY ON Chrysanthemum × grandiflorum (Ramat.) Kitam. IN POLAND, EJPAU 13(4), #27.
Available Online: http://www.ejpau.media.pl/volume13/issue4/abs-27.html

RESULTS OF MUTATION BREEDING ACTIVITY ON CHRYSANTHEMUM × GRANDIFLORUM (RAMAT.) KITAM. IN POLAND

Małgorzata Zalewska¹, Natalia Miler², Alicja Tymoszuk¹, Barbara Drzewiecka³, Janusz Winiecki³
¹ Department of Ornamental Plants and Vegetable Crops, University of Technology and Life Sciences in Bydgoszcz, Poland
² Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
³ Medical Physics Department, Oncology Centre, Bydgoszcz, Poland

ABSTRACT

Our researches concerning somatic mutagenesis in Chrysanthemum × grandiflorum (Ramat.) Kitam. induced with ionizing radiation have been carried out since 1977. First significant results were obtained in vivo when irradiated leaves detached from flowering chrysanthemums were applied. Induction of mutations in subsequent experiments was conducted in vitro with the use of the adventitious buds technique for regeneration. Explants were leaves, callus, internodes and single nodes. Except for callus, all explants were taken from irradiated microcuttings growing in vitro during the mutagenic treatment. The dose of X- and gamma- rays involved in our experiments ranged from 15 to 25 Gy. For X-rays therapeutic apparatus THX-250 Medicor was used. Gamma radiation was obtained from Co⁶⁰ cobalt source generated by Theraton 780 C. Regeneration was conducted on MS medium supplemented with 0.6 mg·dm^-3 BAP and 2.0 mg·dm^-3 IAA. The most spectacular effects were observed when gamma-rays and in vitro regeneration were used. All of mutants were solid, non-mericlinal and non-sectorial chimeras. Our experiments show that over a short period from a single mother cultivar one can obtain numerous attractive mutants, thus creating new cultivar groups.

Key words: Chrysanthemum × grandiflorum (Ramat.) Kitam., mutation breeding, ionizing radiation, in vitro.

Małgorzata Zalewska
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 36
email: zalewska@utp.edu.pl

Natalia Miler
Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22
email: nmiler@utp.edu.pl

Alicja Tymoszuk
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22
email: alicjaskowronek@wp.pl

Barbara Drzewiecka
Medical Physics Department, Oncology Centre,
Bydgoszcz, Poland
Dr I. Romanowskiej 2, 85-796 Bydgoszcz, Poland
phone: (+48) 52 374 34 99
email: basiad@co.bydgoszcz.pl

Janusz Winiecki
Medical Physics Department, Oncology Centre,
Bydgoszcz, Poland
Dr I. Romanowskiej 2, 85-796 Bydgoszcz, Poland
phone: (+48) 52 374 34 99
email: janusz@co.bydgoszcz.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.