Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2010
Volume 13
Issue 4
Topic:
Agronomy
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Tomaszewska-Sowa M. 2010. CYTOMETRIC ANALYSES OF SUGAR BEET (Beta vulgaris L.) PLANTS REGENERATED FROM UNFERTILIZED OVULES CULTURED in vitro, EJPAU 13(4), #09.
Available Online: http://www.ejpau.media.pl/volume13/issue4/abs-09.html

CYTOMETRIC ANALYSES OF SUGAR BEET (BETA VULGARIS L.) PLANTS REGENERATED FROM UNFERTILIZED OVULES CULTURED IN VITRO

Magdalena Tomaszewska-Sowa
Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland

 

ABSTRACT


The results of cytometric analyses of 413 sugar beet plants obtained by regeneration from unfertilized ovules in vitro are presented. The formation of shoots was induced on different MS media varying in the contents and composition of phytohormones. The majority of plants (353) were analyzed only once during the in vitro stage and 60 – twice, during the in vitro stage and also after planting in the field. The analyses of the plants during the in vitro stage revealed that 32.7% were haploids, 45.8% – diploids and 21.5% – mixoploids. The highest percentage of haploid plants was achieved using media containing 4.4 µM BAP and 0.44 µM NAA and 2.2 µM BAP. The repeated analyses of plants (during the in vitro stage and also after planting) show during the in vitro stage the population consisting of 55% haploids and 45% diploids. Repeated tests demonstrated that during the period between the tests some of the haploids underwent a spontaneous diploidization since their percentage was reduced from 55% to 30%. This process was observed only for the plants derived from the media containing kinetin with the addition of NAA or 2.4-D. Observations of regenerants pointed to a vast diversity of phenotypes between haploids and diploids, which can be regarded to as a characteristic feature of plants with the DNA level of 1C and 2C.

Key words: diploids plants, flow cytometry, growth regulators, haploid plants, in vitro culture, ovules, ploidy, sugar beet.


Magdalena Tomaszewska-Sowa
Department of Plant Genetics, Physiology and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland
Bernardyńska 6
85-029 Bydgoszcz
Poland
email: magda@utp.edu.pl

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