Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2010
Volume 13
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Mituła A. , Barszczewski W. , Robak M. 2010. YARROWIA LIPOLYTICA CELL CYCLE STUDY, EJPAU 13(2), #08.
Available Online: http://www.ejpau.media.pl/volume13/issue2/abs-08.html

YARROWIA LIPOLYTICA CELL CYCLE STUDY

Agnieszka Mituła1, Wojciech Barszczewski2, Małgorzata Robak2
1 Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland
2 Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Poland

 

ABSTRACT


Flow cytometry (FC) was used for measuring ploidity and number of cells at each phase of the cell cycle of four Yarrowia lipolytica yeast strains. Synchronization at G0/G1-phase was partially achieved by carbon source starvation, followed by glucose addition. Approximately 10 000 cells were analyzed by measuring the fluorescence of propidium iodine (PI) bound to DNA after 0, 1, 2, 4, 6 hours of glucose addition to the starved cells population. Estimation of cells percentage at each cycle phase was achieved by Cylchred and WinMDI analysis of obtained data. However complete synchronization was not obtained for any of the strains, the generation time representing time interval between
two maxima of cells at S phase was determined. The shortest generation time was noted for Y. lipolytica AWG7 (1h). Comparison of histograms of all tested strains with a haploid type strain roved their haploidy.

Key words: flow cytometry, cell cycle, yeasts, Yarrowia lipolytica.


Agnieszka Mituła
Institute of Biochemistry and Molecular Biology,
University of Wrocław, Poland


Wojciech Barszczewski
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wrocław, Poland
Phone: +48 71 3205463
email: wojciech.barszczewski@up.wroc.pl

Małgorzata Robak
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wrocław, Poland
email: malgorzata.robak@up.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.