EJPAU 2009. Tymoszuk A. , Miler N. , Zalewska M. , Borawska M. THE ROOTING OF CHRYSANTHEMUM [Chrysanthemum × grandiflorum (Ramat.) Kitam] IN in vitro AND in vivo CONDITIONS

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2009
Volume 12
Issue 3

Topic:

Horticulture

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Tymoszuk A. , Miler N. , Zalewska M. , Borawska M. 2009. THE ROOTING OF CHRYSANTHEMUM [Chrysanthemum × grandiflorum (Ramat.) Kitam] IN in vitro AND in vivo CONDITIONS, EJPAU 12(3), #03.
Available Online: http://www.ejpau.media.pl/volume12/issue3/abs-03.html

THE ROOTING OF CHRYSANTHEMUM [CHRYSANTHEMUM × GRANDIFLORUM (RAMAT.) KITAM] IN IN VITRO AND IN VIVO CONDITIONS

Alicja Tymoszuk¹, Natalia Miler², Małgorzata Zalewska¹, Małgorzata Borawska³
¹ Department of Ornamental Plants and Vegetable Crops, University of Technology and Life Sciences in Bydgoszcz, Poland
² Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
³ Department of Ornamental and Vegetable Crops, University of Technology and Life Sciences, Bydgoszcz, Poland

ABSTRACT

The purpose of the present paper is a comparison
of the quality of chrysanthemum plants of three cultivars: 'Bislet', 'Euro' and
'Reddy', which were acclimatized after having been rooted in vitro, with
the quality of plants acclimatized and rooted at the same time in vivo in
selected substrates. In in vitro culture the plants were rooted on MS
medium with an addition of 2mg·dm^-3 IAA, and next acclimatized
in a mixture of peat with perlite in the volume proportion of 2:1 (v:v). Peat,
perlite and a mixture of peat with perlite in the volume proportion of 2:1 (v:v)
were used for direct rooting in vivo taking place simultaneously with
acclimatization. A positive effect of in vivo rooting in perlite on the
increase of the length and weight of the root system was found out. Plants rooted in
vitro had numerous short roots of high weight. The largest fresh weight of
the roots was observed in 'Bislet' and 'Euro' chrysanthemums. 'Bislet' produced
the fewest roots, but they were the longest. In 'Reddy' cv. it was observed that
the roots had the smallest fresh weight and they were the shortest. The highest
increase of the shoot length was observed in the plants previously rooted in
vitro and in the plants rooted in vivo and directly acclimatized in
perlite. The most of new leaves were formed by microcuttings rooted in vitro.

Key words: acclimatization, in vitro rooting, in vivo rooting, Chrysanthemum × grandiflorum (Ramat.) Kitam., substrates.

Alicja Tymoszuk
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22
email: alicjaskowronek@wp.pl

Natalia Miler
Department of Ornamental Plants and Vegetable Crops, Faculty of Agriculture and Biotechnology, University of Science and Technology, Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22
email: nmiler@utp.edu.pl

Małgorzata Zalewska
Department of Ornamental Plants and Vegetable Crops,
University of Technology and Life Sciences in Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 36
email: zalewska@utp.edu.pl

Małgorzata Borawska
Department of Ornamental and Vegetable Crops,
University of Technology and Life Sciences, Bydgoszcz, Poland
Bernardyńska 6, 85-029 Bydgoszcz, Poland
Phone: (+48) 52 374 95 22

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.