Volume 12
Issue 1
Agronomy
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume12/issue1/abs-11.html
PURIFICATION AND PARTIAL CHARACTERIZATION OF PYOVERDINE SYNTHESIZED BY PSEUDOMONAS PUTIDA
Urszula Jankiewicz1, Olga Kuzawińska2
1 Department of Biochemistry, Warsaw University of Life Sciences – SGGW, Poland
2 Department of Biochemistry,
Warsaw University of Life Sciences, Poland
ABSTRACT
A soil strain of Pseudomonas putida intensively
secreted, under iron deficiency conditions, a single form of siderophore-pyoverdine
to the medium. The siderophore was isolated from the culture medium and purified
using chelating chromatography. Purified pyoverdine inhibited the growth of all
studied Fusarium strains except for F. graminearum. The presence
of pyoverdine stimulated the growth of some of the tested strains. Based on MS
spectrum analysis it can be concluded that Pseudomonas putida produced
a pyoverdine with low molecular mass of 800 Da.
Key words: siderophore, pyoverdine, Pseudomonas, biocontrol.
Urszula Jankiewicz
Department of Biochemistry, Warsaw University of Life Sciences – SGGW, Poland
Nowoursynowska 159
02-776 Warsaw
Poland
email: urszula_jankiewicz@sggw.pl
Olga Kuzawińska
Department of Biochemistry,
Warsaw University of Life Sciences, Poland
Nowoursynowska 159, 02-776 Warsaw, Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.