EJPAU 2008. Kwiatkowski M. , Kucharczyk D. , Szczerbowski A. , Łuczyński M. , Mamcarz A. , Jamróz M. OPTIMIZING CONDITIONS FOR ANDROGENESIS INDUCTION IN KOI CARP

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2008
Volume 11
Issue 2

Topic:

Fisheries

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Kwiatkowski M. , Kucharczyk D. , Szczerbowski A. , Łuczyński M. , Mamcarz A. , Jamróz M. 2008. OPTIMIZING CONDITIONS FOR ANDROGENESIS INDUCTION IN KOI CARP, EJPAU 11(2), #09.
Available Online: http://www.ejpau.media.pl/volume11/issue2/abs-09.html

OPTIMIZING CONDITIONS FOR ANDROGENESIS INDUCTION IN KOI CARP

Maciej Kwiatkowski¹, Dariusz Kucharczyk¹, Andrzej Szczerbowski², Marek J. Łuczyński², Andrzej Mamcarz¹, Marta Jamróz¹
¹ Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Poland
² Inland Fisheries Institute, Olsztyn-Kortowo, Poland

ABSTRACT

In aquaculture, such genome manipulations as androgenesis and mitotic gynogenesis are of special interest because the resulting progeny are completely homozygous and carries chromosomes from only one parental source. Androgenesis has a lot of potential applications, inc1uding rapid establishment of inbred lines, analyzing patterns of inheritance and recovery of valuable strains or endangered wild populations or species from cryopreserved sperm. In present work, the optimum conditions of ovarian fluids, thermal shock and UV irradiation to involve androgenesis in koi carp (Cyprinus carpio L.) was checked. The best ovarian fluid was one described for common carp by Bongers et al. [5]. As a semi-lethal heat thermal shock was chosen 2 min lasted at 40°C. The optimum dose of genetic inactivation of koi carp oocytes using UV radiation was 3840 J m^-2.

Key words: androgenesis, artificial ovarian fluid, chromosome set manipulation, koi carp thermal shock, UV irradiation.

Maciej Kwiatkowski
Department of Lake and River Fisheries,
University of Warmia and Mazury in Olsztyn, Poland
Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
email: thornghost@.onet.pl

Dariusz Kucharczyk
Department of Lake and River Fisheries,
University of Warmia and Mazury in Olsztyn, Poland
M. Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Andrzej Szczerbowski
Inland Fisheries Institute,
Olsztyn-Kortowo, Poland
Oczapowskiego 10, 10-719 Olsztyn-Kortowo, Poland

Marek J. Łuczyński
Inland Fisheries Institute,
Olsztyn-Kortowo, Poland
Oczapowskiego 10, 10-719 Olsztyn-Kortowo, Poland

Andrzej Mamcarz
Department of Lake and River Fisheries,
University of Warmia and Mazury in Olsztyn, Poland
M. Oczapowskiego 5, 10-957 Olsztyn, Poland
phone +48 89 523 33 88,
fax +48 89 523 39 69
email: mamcarz@uwm.edu.pl

Marta Jamróz
Department of Lake and River Fisheries,
University of Warmia and Mazury in Olsztyn, Poland
Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.