Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297

Rywińska A. , Wojtatowicz M. , Żarowska B. , Rymowicz W. 2008. BIOSYNTHESIS OF CITRIC ACID BY YEAST YARROWIA LIPOLYTICA A-101-1.31 UNDER REPEATED BATCH CULTIVATION, EJPAU 11(1), #07.
Available Online: http://www.ejpau.media.pl/volume11/issue1/abs-07.html

ABSTRACT

The purpose of the study was to determine growth and acid-forming activities, morphology and physiology of Yarrowia lipolytica acetate mutant A-101-1.31, used for citric acid (CA) production from glucose.

The repeated batch cultivation was carried out in 6 batches. The logarithmic phase of growth was observed only in batch I, while in the other batches, only linear and stationary phases occurred. The volumetric rate of biomass production (Q_X) was decreasing from 0.54 gL^-1h^-1 in batch I to 0.22 gL^-1h^-1 in the last batch. CA was produced at high specific rate (q_CA^g ~ 0.09 ÷ 0.11 gg^-1h^-1; q_CA^n-g ~ 0.04 ÷ 0.05 gg^-1h^-1) and yield (Y_CA^P ~ 0.7 ÷ 0.8 gg^-1) only in four batches, each lasting about 72 hrs. In the other batches, the efficiency of CA synthesis was markedly lower, which was correlated with rapid growth of clones forming S-colonies. Parallely to this phenomenon, a significant increase in the mean volume of cells was observed.

Key words: citric acid, Yarrowia lipolytica, repeated batch culture.

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Anita Rywińska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Chełmońskiego 37/41
51-630 Wrocław
Poland
email: anita.rywinska@wnoz.up.wroc.pl

Maria Wojtatowicz
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
C.K. Norwida 25, 50-375 Wrocław, Poland
Fax. 4871- 3284124
Phone: 48-71-3205117
email: mwojt@wnoz.ar.wroc.pl

Barbara Żarowska
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
C.K. Norwida 25, 50-375 Wrocław, Poland
email: zarowska@ozi.ar.wroc.pl

Waldemar Rymowicz
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroclaw, Poland
Fax. 48-71-3284124
Phone: 48-71-3205143
email: rymowicz@ozi.ar.wroc.pl

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Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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