Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2008
Volume 11
Issue 1
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Sip A. , Dembczyński R. , Białas W. , Juzwa W. , Czaczyk K. , Grajek W. , Jankowski T. 2008. SCALE-UP OF DIVERCIN PRODUCTION BY CARNOBACTERIUM DIVERGENS AS7 IN STIRRED TANK REACTORS, EJPAU 11(1), #04.
Available Online: http://www.ejpau.media.pl/volume11/issue1/abs-04.html

SCALE-UP OF DIVERCIN PRODUCTION BY CARNOBACTERIUM DIVERGENS AS7 IN STIRRED TANK REACTORS

Anna Sip1, Radosław Dembczyński1, Wojciech Białas1, Wojciech Juzwa2, Katarzyna Czaczyk1, Włodzimierz Grajek1, Tomasz Jankowski1
1 Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poland
2 Department of Biotechnology and Food Microbiology, The August Cieszkowski Agricultural University of Poznań, Poland

 

ABSTRACT



Divercin is bacteriocin which is very effective against Listeria sp. In order to study the feasibility of commercialising the production of divercin by Carnobacterium divergens AS7 strain using a large scale bioreactor, a scale-up study from 5 l to 1500 l was carried out using constant agitation power per unit of liquid volume as the scale-up criterion. The maximum specific growth rate as well as the maximum viable cell concentration decreased as the bioreactor scale increased, while enhanced divercin release to the fermentation medium was achieved in 1500 l scale comparing to the reference 5 l bioreactor. This was attributed to nutrient depletion in the large scale bioreactor caused by degradation of the culture medium compounds during the heat sterilisation cycle.

Key words: scale-up, bacteriocin, divercin, fermentation, bioreactor.


Anna Sip
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: +48 061 846 60 04

Radosław Dembczyński
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
phone: +48 61 8466026
email: rdembcz@au.poznan.pl

Wojciech Białas
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
email: wbialas@au.poznan.pl

Wojciech Juzwa
Department of Biotechnology and Food Microbiology,
The August Cieszkowski Agricultural University of Poznań, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland

Katarzyna Czaczyk
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: +48 061 846 60 04


Włodzimierz Grajek
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland

Tomasz Jankowski
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: 061 846 60 04

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.