Volume 11
Issue 1
Biotechnology
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume11/issue1/abs-04.html
SCALE-UP OF DIVERCIN PRODUCTION BY CARNOBACTERIUM DIVERGENS AS7 IN STIRRED TANK REACTORS
Anna Sip1, Radosław Dembczyński1, Wojciech Białas1, Wojciech Juzwa2, Katarzyna Czaczyk1, Włodzimierz Grajek3, Tomasz Jankowski1
1 Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
2 Department of Biotechnology and Food Microbiology,
The August Cieszkowski Agricultural University of Poznań, Poland
3 Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poznań, Poland
ABSTRACT
Divercin is bacteriocin which is very effective against Listeria sp. In order to study the feasibility of commercialising the production of divercin by Carnobacterium divergens AS7 strain using a large scale bioreactor, a scale-up study from 5 l to 1500 l was carried out using constant agitation power per unit of liquid volume as the scale-up criterion. The maximum specific growth rate as well as the maximum viable cell concentration decreased as the bioreactor scale increased, while enhanced divercin release to the fermentation medium was achieved in 1500 l scale comparing to the reference 5 l bioreactor. This was attributed to nutrient depletion in the large scale bioreactor caused by degradation of the culture medium compounds during the heat sterilisation cycle.
Key words: scale-up, bacteriocin, divercin, fermentation, bioreactor.
Anna Sip
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: +48 061 846 60 04
Radosław Dembczyński
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
phone: +48 61 8466026
email: rdembcz@au.poznan.pl
Wojciech Białas
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
email: wbialas@au.poznan.pl
Wojciech Juzwa
Department of Biotechnology and Food Microbiology,
The August Cieszkowski Agricultural University of Poznań, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Katarzyna Czaczyk
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: +48 061 846 60 04
Włodzimierz Grajek
Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poznań, Poland
ul. Wojska Polskiego 48
60-627 Poznań
Poland
Tomasz Jankowski
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: 061 846 60 04
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.