EJPAU 2008. Dziewicka A. , Stawińska N. , Kochanowska I. OSTEOLITIC ACTIVITY OF OSTEOCLAST-LIKE CELLS DERIVED FROM PERIPHERAL BLOOD OF DIFFERENT SPECIES

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2008
Volume 11
Issue 1

Topic:

Veterinary Medicine

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Dziewicka A. , Stawińska N. , Kochanowska I. 2008. OSTEOLITIC ACTIVITY OF OSTEOCLAST-LIKE CELLS DERIVED FROM PERIPHERAL BLOOD OF DIFFERENT SPECIES, EJPAU 11(1), #03.
Available Online: http://www.ejpau.media.pl/volume11/issue1/abs-03.html

OSTEOLITIC ACTIVITY OF OSTEOCLAST-LIKE CELLS DERIVED FROM PERIPHERAL BLOOD OF DIFFERENT SPECIES

Agnieszka Dziewicka¹, Natalia Stawińska², Iwona Ewa Kochanowska¹
¹ Department of Experimental Therapy, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
² Department of Periodontology, University of Medicine, Wroclaw, Poland

ABSTRACT

Osteolitic activity of human osteoclasts precursors derived from peripheral blood mononuclear cells (PBMC) was demonstrated several times by many investigators. However, there is insufficient knowledge about osteoclast’s precursors from PBMC of different species (mouse, rat, rabbit, bovine and guinea pig). Digestive activity of this cells manifests in their ability to bone collagen degradation in vitro in absence of stromal cells and without addition of growth factors, cytokines and steroids. The major markers generally used to identify osteoclasts are tartrate resistant acid phosphatase (TRAP) and calcitonin receptor (CTR) can be determinate by RT-PCR technique. C-telopeptides, markers of bone turnover caused by osteoclastic bone digestion, were determined with ELISA technique in supernatants. It was observed, that percentage of TRAP-positive cells in PBMC fraction differs in investigated species. Digestive properties of pre-osteoclasts manifested in amount of released C-telopeptides (CTx) depend on time of propagation and amount of propagated cells. TRAP and CTR genes expressions were detected in pre-osteoclasts of investigated species. Osteolitic properties of pre-osteoclasts derived from PBMC appear independently from addition of growth factors and cytokines and also without contact with initiating osteoclastogenesis cells like osteoblasts or stromal cells. High density cultures of pre-osteoclasts occur sufficient for successful bone digestion.

Key words: calcitonine receptor, C-telopeptides, osteoclast-like cells, peripheral blood mononuclear cells, pre-osteoclasts, tartrate resistant acid phosphatase.

Agnieszka Dziewicka
Department of Experimental Therapy,
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy,
Polish Academy of Sciences
Weigla 12 St., 53-114 Wroclaw, Poland
Phone: 071 370 99 17
email: dziewicka@iitd.pan.wroc.pl

Natalia Stawińska
Department of Periodontology,
University of Medicine, Wroclaw, Poland
Krakowska 26 St., 50-425 Wroclaw, Poland
Phone: 071 784 03 83
email: nstawin@wp.pl

Iwona Ewa Kochanowska
Department of Experimental Therapy,
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy,
Polish Academy of Sciences
Weigla 12 St., 53-114 Wroclaw, Poland
Phone: 071 370 99 66
email: kochanie@iitd.pan.wroc.pl

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.