Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2007
Volume 10
Issue 4
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Robak M. , Rymowicz W. , Filipkowski P. 2007. EFFECT OF SODIUM ACETATE ON CITRIC ACID PRODUCTION FROM GLUCOSE BY YARROWIA LIPOLYTICA, EJPAU 10(4), #22.
Available Online: http://www.ejpau.media.pl/volume10/issue4/abs-22.html

EFFECT OF SODIUM ACETATE ON CITRIC ACID PRODUCTION FROM GLUCOSE BY YARROWIA LIPOLYTICA

Małgorzata Robak1, Waldemar Rymowicz1, Paweł Filipkowski2
1 Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Poland
2 Department of Food Chemistry, Technology and Biotechnology, Gdansk University of Technology, Poland

 

ABSTRACT



The effect of sodium acetate addition on citric acid (CA) biosynthesis in batch fermentation process from glucose by acetate non utilizing (acu-) mutant Yarrowia lipolytica A-101.1.31 was studied. Acetate as co-substrate in glucose medium, has improved the specific production rate in the first 24 hours of process, yield and productivity. Among the tested doses (1, 2, 8, 16, 20 gL-1) the addition of 2 gL-1 of sodium acetate had the most positive effect on the CA biosynthesis by Y.lipolytica A-101.1.31. In the future, a study on a continuous addition of sodium acetate could be taken into consideration because acetate was rapidly metabolized.

Key words: Yarrowia lipolytica, acetate utilisation, citrate production .


Małgorzata Robak
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wrocław, Poland
email: malgorzata.robak@up.wroc.pl

Waldemar Rymowicz
Department of Biotechnology and Food Microbiology,
Wrocław University of Environmental and Life Sciences, Poland
Norwida 25, 50-373 Wroclaw, Poland
Fax. 48-71-3284124
Phone: 48-71-3205143
email: rymowicz@ozi.ar.wroc.pl

Paweł Filipkowski
Department of Food Chemistry, Technology and Biotechnology,
Gdansk University of Technology, Poland
Narutowicza 11/12, 80-952 Gdansk, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.