EJPAU 2007. Wessely-Szponder J. THE EFFECT OF TNFα ON THE PRODUCTION OF NITRIC OXIDE BY NEUTROPHILS ISOLATED FROM HEIFERS IN THE COURSE OF BOVINE RESPIRATORY DISEASE

Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.

2007
Volume 10
Issue 4

Topic:

Veterinary Medicine

ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES

Wessely-Szponder J. 2007. THE EFFECT OF TNFα ON THE PRODUCTION OF NITRIC OXIDE BY NEUTROPHILS ISOLATED FROM HEIFERS IN THE COURSE OF BOVINE RESPIRATORY DISEASE, EJPAU 10(4), #21.
Available Online: http://www.ejpau.media.pl/volume10/issue4/abs-21.html

THE EFFECT OF TNFα ON THE PRODUCTION OF NITRIC OXIDE BY NEUTROPHILS ISOLATED FROM HEIFERS IN THE COURSE OF BOVINE RESPIRATORY DISEASE

Joanna Wessely-Szponder
Department of Pathophysiology, Faculty of Veterinary Medicine, Agricultural University in Lublin, Poland

ABSTRACT

In the course of Bovine Respiratory Disease (BRD), lung injury is caused, among others, by neutrophil products, including nitric oxide (NO). Destructive action of this free radical on respiratory tract tissues is stimulated by some cytokines. The purpose of this study was to determine how TNFα influences on NO generation by neutrophils in the course of BRD. This study revealed that neutrophils isolated from heifers with acute BRD produced greater amounts of NO than in chronic BRD. In all phases of BRD and in both forms of the disease the concentration of TNFα in the range of 0.05 ng·ml^-1 to 0.5 ng·ml^-1 had a stimulatory effect on neutrophils after 0.5 h and 24 h incubation. Moreover, the greatest NO production was observed in cultures of neutrophils from an acute BRD in the concentration of 0.5 ng·ml^-1 after 0.5 h and 24h incubation. After 48h incubation NO production increased only at a concentration of 0.05 ng·ml^-1. Above these concentrations production of NO decreased. The most pronounced inhibitory action of TNFα on generation of NO by neutrophils became in acute BRD in cultures after 0.5 h at 50 ng·ml^-1, and after 24 h at 5 ng·ml^-1, and was related to diminished viability of neutrophils.

Key words: Bovine Respiratory Disease, TNFα, nitric oxide, neutrophil.

Joanna Wessely-Szponder
Department of Pathophysiology,
Faculty of Veterinary Medicine,
Agricultural University in Lublin, Poland
Akademicka 12, 20-033 Lublin, Poland
email: joanna.wessely@up.lublin.pl

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