Volume 10
Issue 3
Animal Husbandry
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Available Online: http://www.ejpau.media.pl/volume10/issue3/abs-16.html
RACCOON DOG (NYCTEREUTES PROCYONOIDES GRAY) PARENTAGE TESTING BASED ON STR MARKERS
Brygida Ślaska1, Grzegorz Zięba1, Grażyna Jeżewska-Witkowska2
1 Department of Biological Bases of Animal Production,
University of Agriculture, Lublin, Poland
2 Department of Biological Basis of Animal Production, University of Life Sciences in Lublin, Poland
ABSTRACT
We presented a practical and efficient parentage analysis test for raccoon dogs. The method makes use of 15 polymorphic microsatellite markers. Parentage control was performed for 3 generations of raccoon dog (207 animals altogether) by amplification of microsatellites. Exclusion probabilities ranging from 0.959 assuming one known parent to 0.998 assuming both known parents, were attained. It is possible to use the set of 15 markers in this study for routine parentage testing, but further research should be done to examine whether the set are practically effective. Microsatellite markers are useful tools for parentage testing in raccoon dogs.
Key words: Canidae, microsatellites, parentage testing, raccoon dog.
Brygida Ślaska
Department of Biological Bases of Animal Production,
University of Agriculture, Lublin, Poland
Akademicka 13, Lublin 20-950, Poland
Phone: +48 81 445-66-28
email: brygida.slaska@ar.lublin.pl
Grzegorz Zięba
Department of Biological Bases of Animal Production,
University of Agriculture, Lublin, Poland
Akademicka 13, Lublin 20-950, Poland
Grażyna Jeżewska-Witkowska
Department of Biological Basis of Animal Production, University of Life Sciences in Lublin, Poland
13 Akademicka St., 20-950 Lublin, Poland
Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.