Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2007
Volume 10
Issue 3
Topic:
Veterinary Medicine
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Kupczyński R. , Cupok A. 2007. SENSITIVITY AND SPECIFICITY OF VARIOUS TESTS DETERMINING β-HYDROXYBUTYRATE ACID IN DIAGNOSIS OF KETOSIS IN COWS, EJPAU 10(3), #15.
Available Online: http://www.ejpau.media.pl/volume10/issue3/abs-15.html

SENSITIVITY AND SPECIFICITY OF VARIOUS TESTS DETERMINING β-HYDROXYBUTYRATE ACID IN DIAGNOSIS OF KETOSIS IN COWS

Robert Kupczyński1, Agnieszka Cupok2
1 Department of Environmental Hygiene and Animal Welfare, Wroc³aw University of Environmental and Life Sciences, Poland
2 Department of Animal Hygiene and Ichthyology, Wroc³aw University of Environmental and Life Sciences, Poland

 

ABSTRACT



The objective of the study was to compare rapid field tests used for the determination of β-hydroxybutyrate acid or glucose concentration in the diagnosis of sub-clinical ketosis in cows. β-hydroxybutyrate acid (BHBA) concentration in milk was determined with the use of Keto-TestTM strip test, while BHBA and glucose in full blood were determined by means of MediSense Optium glucometer. These parameters were also determined with an analytical, enzymatic and oxidase method. Mean BHBA and glucose concentration in blood plasma amounted to 715.04 μmol·dm3 and 3.39 mmol·dm3 (analytically), and in full blood 670.83 μmol·dm3 and 3.50 mmol·dm3 (glucometer), respectively. The usefulness of MediSense Optium glucometer for the diagnosis of sub-clinical ketosis in individual cows as well as the herd-based was found. Sensitivity and specificity of the glucometer was 100% (cut-point >1400 µmol·dm3 BHBA). Sensitivity and specificity of Keto-Test were lower. The test should be used in individual cows. Statistically high correlation (0.92 and 0.78; p<0.01) between BHBA concentration and glucose determined with glucometer (full blood) and analytical method (blood plasma) was found.

Key words: sub-clinical ketosis, rapid test, glucometer, β–hydroxybutyrate.


Robert Kupczyński
Department of Environmental Hygiene and Animal Welfare,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³mońskiego 38 C, 51-630 Wroc³aw, Poland
Phone: (+48 71) 320 59 41
email: robert.kupczynski@up.wroc.pl

Agnieszka Cupok
Department of Animal Hygiene and Ichthyology,
Wroc³aw University of Environmental and Life Sciences, Poland
Che³monskiego 38 C. 51-630 Wroc³aw. Poland
Phone: (+4871) 320 59 41

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