Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2007
Volume 10
Issue 3
Topic:
Veterinary Medicine
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Zimecki M. , Kocięba M. , Weber-D±browska B. , Łusiak-Szelachowska M. , Syper D. , Górski A. 2007. EFFECTS OF BACTERIOPHAGES ON CLEARANCE OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS AND SERUM CYTOKINE LEVELS IN INFECTED MICE, EJPAU 10(3), #06.
Available Online: http://www.ejpau.media.pl/volume10/issue3/abs-06.html

EFFECTS OF BACTERIOPHAGES ON CLEARANCE OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS AND SERUM CYTOKINE LEVELS IN INFECTED MICE

Michał Zimecki, Maja Kocięba, Beata Weber-D±browska, Marzanna Łusiak-Szelachowska, Danuta Syper, Andrzej Górski
Institute of Immunology and Experimental Therapy of Polish Academy of Sciences, Wrocław, Poland

 

ABSTRACT



In this study we demonstrated that concomitant, intraperitoneal administration of T8 and A5 bacteriophages with lethal doses of Pseudomonas aeruginosa or Staphylococcus aureus, respectively, protected CBA mice against lethality and significantly reduced the number of CFUs in the organs 24h after infection. In the case of P. aeruginosa infection, the serum levels of tumor necrosis factor alpha and interleukin 6 were not significantly altered by bacteriophages. On the other hand, the levels of these cytokines were significantly elevated upon administration of bacteriophages during S. aureus infection. The relevance of the phage-induced changes in the cytokine levels to the protection of mice against Gram-negative and Gram-positive sepsis is discussed.

Key words: bacteriophages, Pseudomonas aeruginosa, Staphylococcus aureus, mice, TNF-alpha, interleukin-6.

Michał Zimecki
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland
email: zimecki@immuno.iitd.pan.wroc.pl

Maja Kocięba
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland

Beata Weber-D±browska
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland
Phone: (+4871) 3371172
Fax: (+4871) 3371382
email: weber@iitd.pan.wroc.pl

Marzanna Łusiak-Szelachowska
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland


Danuta Syper
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland


Andrzej Górski
Institute of Immunology and Experimental Therapy
of Polish Academy of Sciences, Wrocław, Poland
R. Weigla 12, 53-114 Wrocław, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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