Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2007
Volume 10
Issue 3
Topic:
Veterinary Medicine
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Madej J. , Boratyński J. , Nowacki W. 2007. SIMILARITY OF VITAMIN D-BINDING PROTEIN (DBP) BETWEEN VARIOUS ANIMAL SPECIES IN DOUBLE IMMUNODIFFUSION AND WESTERN BLOTTING, EJPAU 10(3), #02.
Available Online: http://www.ejpau.media.pl/volume10/issue3/abs-02.html

SIMILARITY OF VITAMIN D-BINDING PROTEIN (DBP) BETWEEN VARIOUS ANIMAL SPECIES IN DOUBLE IMMUNODIFFUSION AND WESTERN BLOTTING

Jan P. Madej1, Janusz Boratyński2, Wojciech Nowacki1
1 Department of Veterinary Prevention and Immunology, Department and Clinic of Obsterics, Ruminant Diseases and Animal Health Care, Wroclaw University of Environmental Sciences, Poland
2 Department of Experimental Oncology, Institute of Immunology and Experimental Therapy of the Polish Academy of Science, Wroc³aw, Poland

 

ABSTRACT



For the purpose of the study, self-produced polyclonal rabbit anti-DBP serum as well as sera from various animal species were used. In double immunodiffusion, a cross-reaction was demonstrated between human anti-DBP serum and sera from all the remaining animals: horse, pig, dog, cattle, cat, mouse, rat, sheep, goat. The character and intensity of the ongoing precipitation reaction were also determined. The strong cross-reaction was observed with dog and cat serum, intermediate – with pig, horse and mouse, and weak with cattle, sheep, goat and rat. The molecular mass of DBP in selected species was assessed using SDS-PAGE followed by western blotting and was estimated in horse, cat and goat sera as 51 kDa, in dog and cattle sera – 49 kDa, in human serum – 48 kDa, in pig serum – 47 kDa, in rat and mouse sera – 44 kDa).

Key words: vitamin D-binding protein, DBP, Gc globulin, double immunodiffusion, animals.

Jan P. Madej
Department of Veterinary Prevention and Immunology,
Department and Clinic of Obsterics, Ruminant Diseases and Animal Health Care,
Wroclaw University of Environmental Sciences, Poland
Norwida 31, 50-375 Wroc³aw, Poland
email: jan_madej@interia.pl

Janusz Boratyński
Department of Experimental Oncology,
Institute of Immunology and Experimental Therapy
of the Polish Academy of Science, Wroc³aw, Poland
Weigla 12, 53-114 Wroc³aw, Poland
Jan Dlugosz, Czestochowa University,
Institute of Chemistry and Environmental Protection,
Al. Armii Krajowej 13/15, 42-201 Czestochowa, Poland

Wojciech Nowacki
Department of Veterinary Prevention and Immunology,
Department and Clinic of Obsterics, Ruminant Diseases and Animal Health Care,
Wroclaw University of Environmental Sciences, Poland
Norwida 31, 50-375 Wroc³aw, Poland

Responses to this article, comments are invited and should be submitted within three months of the publication of the article. If accepted for publication, they will be published in the chapter headed 'Discussions' and hyperlinked to the article.

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