Electronic Journal of Polish Agricultural Universities (EJPAU) founded by all Polish Agriculture Universities presents original papers and review articles relevant to all aspects of agricultural sciences. It is target for persons working both in science and industry,regulatory agencies or teaching in agricultural sector. Covered by IFIS Publishing (Food Science and Technology Abstracts), ELSEVIER Science - Food Science and Technology Program, CAS USA (Chemical Abstracts), CABI Publishing UK and ALPSP (Association of Learned and Professional Society Publisher - full membership). Presented in the Master List of Thomson ISI.
2007
Volume 10
Issue 2
Topic:
Biotechnology
ELECTRONIC
JOURNAL OF
POLISH
AGRICULTURAL
UNIVERSITIES
Białas W. , Jankowski T. 2007. HIGH-PRESSURE HOMOGENISATION OF BAKER’S YEAST FOR THE SELECTIVE RECOVERY OF INVERTASE, EJPAU 10(2), #03.
Available Online: http://www.ejpau.media.pl/volume10/issue2/abs-03.html

HIGH-PRESSURE HOMOGENISATION OF BAKER’S YEAST FOR THE SELECTIVE RECOVERY OF INVERTASE

Wojciech Białas, Tomasz Jankowski
Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poland

 

ABSTRACT



The process of yeast cell disruption using a high-pressure double-valve pilot-scale homogeniser has been investigated for the selective release of invertase. The first-order kinetics was applied for the determination of the release rate of invertase and total proteins. The release rate of invertase was higher than that of total proteins indicating this enzyme to be preferentially located outside the yeast cell membrane. It was found that the best conditions of the disruption process of the yeast cells were achieved for the operating pressure of 50 MPa and 10 passes of the cell suspension through the homogeniser. Increasing the disruption pressure above 50 MPa decreased the selectivity of invertase release, while extending the number of passes caused more shear damage to the enzyme.

Key words: cell disruption, homogeniser, yeast, invertase, location factor.


Wojciech Białas
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
email: wbialas@au.poznan.pl

Tomasz Jankowski
Department of Biotechnology and Food Microbiology,
Poznań University of Life Sciences, Poland
Wojska Polskiego 48, 60-627 Poznań, Poland
Phone: 061 846 60 04

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